PMID- 1453486
OWN - NLM
STAT- MEDLINE
DCOM- 19930106
LR  - 20031114
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 33
IP  - 1
DP  - 1992 Sep
TI  - Nerve growth factor (NGF) receptors in a central nervous system glial cell line: 
      upregulation by NGF and brain-derived neurotrophic factor.
PG  - 82-90
AB  - The neurotrophic proteins nerve growth factor (NGF) and brain-derived 
      neurotrophic factor (BDNF) are related in their primary amino acid structures. In 
      this study we investigated the extent to which the low-affinity NGF receptor 
      (LNGFR) in C6 glioma cells can discriminate between the neurotrophins NGF and 
      BDNF. LNGFR-immunoreactivity (IR) was studied in C6 cells treated for 16 hr with 
      NGF (50 ng/ml) or BDNF (10 ng/ml), using immunogold labelling and electron 
      microscopic morphometric analysis. The cells were exposed to the anti-NGFR 
      antibody 192-IgG, followed by immunoglobulin conjugated with colloidal gold. 
      Untreated C6 cells exhibited some surface gold label (positive LNGFR-IR). Cells 
      treated with NGF or BDNF displayed significantly increased LNGFR-IR on all 
      surfaces in terms of gold labeling, which was more pronounced in NGF-treated 
      cells. LNGFR-IR was also localized in coated endocytotic vesicles, in smooth 
      endoplasmic reticulum, and in secondary multivesicular lysosomes in 
      neurotrophin-treated and untreated cells. The increase in LNGFR protein was 
      further substantiated by a correspondingly higher content of LNGFR mRNA detected 
      after 15 hr of either NGF or BDNF treatment. These results suggest that the LNGFR 
      in glial cells can be upregulated by the structurally related neurotrophins NGF 
      and BDNF.
FAU - Spoerri, P E
AU  - Spoerri PE
AD  - Department of Cellular Biology, Fidia Research Laboratories, Abano Terme, Italy.
FAU - Romanello, S
AU  - Romanello S
FAU - Petrelli, L
AU  - Petrelli L
FAU - Negro, A
AU  - Negro A
FAU - Dal Toso, R
AU  - Dal Toso R
FAU - Leon, A
AU  - Leon A
FAU - Skaper, S D
AU  - Skaper SD
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Brain Neoplasms/metabolism
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Line
MH  - Glioma/metabolism
MH  - Iodine Radioisotopes
MH  - Microscopy, Electron
MH  - Microscopy, Immunoelectron
MH  - Nerve Growth Factors/*pharmacology
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Neuroglia/*metabolism
MH  - Rats
MH  - Receptors, Nerve Growth Factor/*metabolism
MH  - Tumor Cells, Cultured
MH  - Up-Regulation/*drug effects
EDAT- 1992/09/01 00:00
MHDA- 1992/09/01 00:01
CRDT- 1992/09/01 00:00
PHST- 1992/09/01 00:00 [pubmed]
PHST- 1992/09/01 00:01 [medline]
PHST- 1992/09/01 00:00 [entrez]
AID - 10.1002/jnr.490330111 [doi]
PST - ppublish
SO  - J Neurosci Res. 1992 Sep;33(1):82-90. doi: 10.1002/jnr.490330111.