PMID- 1454682
OWN - NLM
STAT- MEDLINE
DCOM- 19930107
LR  - 20200313
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 71
IP  - 10
DP  - 1992 Oct
TI  - Colostrum from cows immunized with Eimeria acervulina antigens reduces parasite 
      development in vivo and in vitro.
PG  - 1637-45
AB  - Experiments were undertaken to determine whether passive immunization utilizing 
      hyperimmune bovine colostrum (HBC) specific for Eimeria acervulina (EA) antigens 
      conferred protection against coccidiosis in chickens. The HBC was produced by 
      immunizing three pregnant, nonmilking Jersey cows with EA antigens administered 
      via one intramuscular injection followed by three intramammary infusions at 
      approximately 10, 8, 6, and 4 wk before parturition. One cow was immunized with 
      sporozoites (SZ), the second with merozoites (MZ), and the third with recombinant 
      merozoite antigen (rMZ). A fourth cow, unimmunized, provided normal colostrum 
      (NC) for control purposes. Colostral whey from each cow was tested by ELISA for 
      antibody against SZ, MZ, and rMZ antigens. In all immunized cows, antiparasite 
      titers were elevated above those of the control. Antibodies from MZ- and 
      rMZ-immunized cows recognized both MZ and rMZ antigen. Separate groups of 
      2-wk-old chickens received two oral doses of anti-SZ, -MZ, or -rMZ HBC or NC or 
      PBS daily from 1 day before through 6 days after oral inoculation (DAI) with EA 
      oocysts. Feces from each group were examined for oocysts. Intestines were 
      examined for lesions 6 DAI. Histologic sections of duodenum were examined for 
      asexual stages and gametocytes utilizing monoclonal antibody and fluorescence 
      microscopy. In Experiments 1 and 2, oocyst production was reduced in all 
      HBC-treated groups, except one treated with rMZ HBC, compared with PBS- or 
      NC-treated groups. In Experiment 2, the severity of lesions was significantly 
      reduced in all HBC-treated groups compared with those that received NC or PBS. 
      Significantly fewer developmental stages were found in histological sections from 
      all chickens treated with anti-SZ and anti-rMZ HBC than from controls. Anti-SZ 
      HBC significantly reduced the number of intracellular SZ found 24 h after their 
      inoculation into cultures of primary chicken kidney cells. These results suggest 
      that HBC specific for certain EA antigens can inhibit parasite development and 
      reduce severity of parasite-related gut lesions.
FAU - Fayer, R
AU  - Fayer R
AD  - Zoonotic Diseases Laboratory, Beltsville Agricultural Research Center, Maryland 
      20705-2350.
FAU - Jenkins, M C
AU  - Jenkins MC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Antigens, Protozoan)
SB  - IM
MH  - Animals
MH  - Antibodies, Protozoan/analysis
MH  - Antigens, Protozoan/*immunology
MH  - Cattle/*immunology
MH  - Chickens/*immunology/parasitology
MH  - Coccidiosis/parasitology/prevention & control/*veterinary
MH  - Colostrum/*immunology
MH  - Duodenum/parasitology
MH  - Eimeria/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - *Immunization, Passive
MH  - Poultry Diseases/parasitology/*prevention & control
EDAT- 1992/10/01 00:00
MHDA- 1992/10/01 00:01
CRDT- 1992/10/01 00:00
PHST- 1992/10/01 00:00 [pubmed]
PHST- 1992/10/01 00:01 [medline]
PHST- 1992/10/01 00:00 [entrez]
AID - S0032-5791(19)33713-7 [pii]
AID - 10.3382/ps.0711637 [doi]
PST - ppublish
SO  - Poult Sci. 1992 Oct;71(10):1637-45. doi: 10.3382/ps.0711637.