PMID- 14550914 OWN - NLM STAT- MEDLINE DCOM- 20031110 LR - 20190701 IS - 0304-3940 (Print) IS - 0304-3940 (Linking) VI - 350 IP - 3 DP - 2003 Oct 30 TI - Effect of early thyroxine treatment on brain-derived neurotrophic factor mRNA expression and protein amount in the rat medial septum/diagonal band of Broca. PG - 141-4 AB - There is evidence that morphological alterations concerning deficiency or abundance of thyroid hormones (TH) may be mediated by brain-derived neurotrophic factor (BDNF). It has been demonstrated that the mRNA-expression of BDNF is increased after TH-treatment during the first postnatal weeks. After transient treatment of newborn rats with thyroxine mRNA expression, protein concentration and number and size of BDNF-immunopositive neurons were quantified in the medial septum/vertical diagonal Band of Broca (MS/vDB). The number and size of BDNF-immunopositive neurons were estimated in young (P10) and adult (4 months). The amount of mRNA and protein are significantly increased in TH-treated rats at P10 compared to control animals. TH-treated animals showed a significant decrease of BDNF-immunopositive cell numbers in the adulthood. The results demonstrate a correlated increase of BDNF mRNA and protein in the septum at P10 which is an important stage of differentiation processes in the septohippocampal system. These results provide further evidence that BDNF is a possible candidate for the mediation the TH effects in the MS/vDB. FAU - Camboni, Daniele AU - Camboni D AD - Institute of Anatomy, Otto-von-Guericke-University, Leipziger Strasse 44, D-39120 Magdeburg, Germany. FAU - Roskoden, Thomas AU - Roskoden T FAU - Schwegler, Herbert AU - Schwegler H LA - eng PT - Comparative Study PT - Journal Article PL - Ireland TA - Neurosci Lett JT - Neuroscience letters JID - 7600130 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (RNA, Messenger) RN - Q51BO43MG4 (Thyroxine) SB - IM MH - Age Factors MH - Animals MH - Animals, Newborn MH - Brain-Derived Neurotrophic Factor/*biosynthesis/drug effects/genetics MH - Diagonal Band of Broca/drug effects/*metabolism MH - Gene Expression Regulation MH - Immunohistochemistry MH - Neurons/drug effects/*metabolism MH - RNA, Messenger/analysis MH - Rats MH - Rats, Wistar MH - Reverse Transcriptase Polymerase Chain Reaction MH - Thyroxine/*pharmacology EDAT- 2003/10/11 05:00 MHDA- 2003/11/11 05:00 CRDT- 2003/10/11 05:00 PHST- 2003/10/11 05:00 [pubmed] PHST- 2003/11/11 05:00 [medline] PHST- 2003/10/11 05:00 [entrez] AID - S0304394003008802 [pii] AID - 10.1016/s0304-3940(03)00880-2 [doi] PST - ppublish SO - Neurosci Lett. 2003 Oct 30;350(3):141-4. doi: 10.1016/s0304-3940(03)00880-2.