PMID- 14551736
OWN - NLM
STAT- MEDLINE
DCOM- 20040309
LR  - 20131121
IS  - 0344-5704 (Print)
IS  - 0344-5704 (Linking)
VI  - 53
IP  - 2
DP  - 2004 Feb
TI  - Antitumoral properties of two new vanadyl(IV) complexes in osteoblasts in 
      culture: role of apoptosis and oxidative stress.
PG  - 163-72
AB  - BACKGROUND: Vanadium derivatives have been reported to display different 
      biological effects, and in particular antineoplastic activity has been 
      demonstrated in both in vivo and in vitro studies. PURPOSE. To study the effect 
      of two new organic vanadyl(IV) complexes (one with glucose, GluVO, and the other 
      with naproxen, NapVO) in osteosarcoma cells. METHODS: UMR106 osteosarcoma cells 
      and, for comparison, nontransformed MC3T3E1 osteoblasts were used. Proliferation 
      and differentiation were assessed using the crystal violet assay and ALP specific 
      activity, respectively. Morphological alterations were assessed by light 
      microscopy. Lipid peroxidation was evaluated in terms of production of 
      thiobarbituric acid-reactive substances (TBARS) and apoptosis was measured using 
      annexin V. Extracellular regulated kinase (Erk) activation was investigated by 
      Western blotting. RESULTS: Vanadium complexes caused morphological alterations 
      and they strongly inhibited UMR106 cell proliferation and differentiation. In 
      contrast, in MC3T3E1 cells, these vanadium derivatives had a relatively weak 
      action. In UMR106 tumoral cells there was a significant increase in TBARS 
      production. Both vanadium complexes induced apoptosis and activation of Erk. 
      PD98059, an inhibitor of Erk phosphorylation, did not block the vanadium-induced 
      antitumoral action. However, the antioxidants vitamins C and E abrogated the 
      apoptosis and TBARS production induced by the vanadium complexes. CONCLUSIONS: 
      GluVO and NapVO exerted an antitumoral effect in UM106 osteosarcoma cells. They 
      inhibited cell proliferation and differentiation. While the Erk cascade seems not 
      to be directly related to the bioactivity of these vanadium derivatives, the 
      action of both vanadium complexes with organic ligands may be mediated by 
      apoptosis and oxidative stress.
FAU - Molinuevo, Maria S
AU  - Molinuevo MS
AD  - Catedra de Bioquimica Patologica, Facultad de Ciencias Exactas, Universidad 
      Nacional de La Plata, 47 y 115, 1900 La Plata, Argentina.
FAU - Barrio, Daniel A
AU  - Barrio DA
FAU - Cortizo, Ana M
AU  - Cortizo AM
FAU - Etcheverry, Susana B
AU  - Etcheverry SB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031009
PL  - Germany
TA  - Cancer Chemother Pharmacol
JT  - Cancer chemotherapy and pharmacology
JID - 7806519
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Vanadium Compounds)
RN  - 57Y76R9ATQ (Naproxen)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/pharmacology
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/drug effects
MH  - Blotting, Western
MH  - Cell Differentiation/drug effects
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Glucose/pharmacology
MH  - Lipid Peroxidation/drug effects
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Mitotic Index
MH  - Naproxen/pharmacology
MH  - Osteoblasts/*drug effects
MH  - Oxidation-Reduction
MH  - Oxidative Stress/drug effects
MH  - Vanadium Compounds/*pharmacology
EDAT- 2003/10/11 05:00
MHDA- 2004/03/10 05:00
CRDT- 2003/10/11 05:00
PHST- 2003/04/15 00:00 [received]
PHST- 2003/08/05 00:00 [accepted]
PHST- 2003/10/11 05:00 [pubmed]
PHST- 2004/03/10 05:00 [medline]
PHST- 2003/10/11 05:00 [entrez]
AID - 10.1007/s00280-003-0708-7 [doi]
PST - ppublish
SO  - Cancer Chemother Pharmacol. 2004 Feb;53(2):163-72. doi: 
      10.1007/s00280-003-0708-7. Epub 2003 Oct 9.