PMID- 14552593
OWN - NLM
STAT- MEDLINE
DCOM- 20040114
LR  - 20190917
IS  - 1120-6721 (Print)
IS  - 1120-6721 (Linking)
VI  - 13
IP  - 7
DP  - 2003 Aug-Sep
TI  - Tumor necrosis factor-alpha (TNF-alpha) in seasonal allergic conjunctivitis and 
      vernal keratoconjunctivitis.
PG  - 606-10
AB  - PURPOSE: To quantify the presence of the proinflammatory cytokine tumor necrosis 
      factor-alpha (TNF-alpha) in allergic conjunctivitis. MATERIALS AND METHODS: Tears 
      and peripheral blood samples were collected from patients with seasonal allergic 
      conjunctivitis (SAC, n=6), vernal keratoconjunctivitis (VKC, n=12), and normal 
      subjects (CT, n=12). From an additional six nonactive allergic patients, tears 
      were collected before and after specific conjunctival allergen challenge (CAC). 
      Upper tarsal conjunctival biopsies were obtained from five CT and five VKC 
      patients. TNF-alpha in tears was measured by enzyme-linked immunoassay and 
      identified in tissues by immunohistochemistry. RESULTS: Tear TNF-alpha levels in 
      VKC patients were significantly increased compared to CT (p=0.03), and were 
      significantly correlated with the severity of the disease. No differences were 
      found between SAC and CT tear samples. TNF-alpha serum levels were higher in VKC 
      than CT, however, this difference was not statistically significant. After CAC, 
      tear TNF-alpha levels were found increased in only one of six patients. In VKC 
      tissues, TNF-alpha positive cells were significantly increased compared to CT 
      (p=0.03). CONCLUSIONS: TNF-alpha may have a significant role in severe forms of 
      allergic conjunctivitis.
FAU - Leonardi, A
AU  - Leonardi A
AD  - Department of Neuroscience, Ophthalmology and Ocular Inflammation Unit, 
      University of Padova, Padova, Italy. andrea.leonardi@unipd.it
FAU - Brun, P
AU  - Brun P
FAU - Tavolato, M
AU  - Tavolato M
FAU - Plebani, M
AU  - Plebani M
FAU - Abatangelo, G
AU  - Abatangelo G
FAU - Secchi, A G
AU  - Secchi AG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Eur J Ophthalmol
JT  - European journal of ophthalmology
JID - 9110772
RN  - 0 (Allergens)
RN  - 0 (Biomarkers)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Allergens/adverse effects
MH  - Biomarkers/analysis
MH  - Child
MH  - Conjunctivitis, Allergic/*blood/etiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Male
MH  - Seasons
MH  - Tears/*metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2003/10/14 05:00
MHDA- 2004/01/15 05:00
CRDT- 2003/10/14 05:00
PHST- 2003/10/14 05:00 [pubmed]
PHST- 2004/01/15 05:00 [medline]
PHST- 2003/10/14 05:00 [entrez]
AID - 10.1177/112067210301300702 [doi]
PST - ppublish
SO  - Eur J Ophthalmol. 2003 Aug-Sep;13(7):606-10. doi: 10.1177/112067210301300702.