PMID- 14553950
OWN - NLM
STAT- MEDLINE
DCOM- 20031103
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 146
IP  - 2
DP  - 2003 Oct 15
TI  - Interphase cytogenetic analysis in Argentinean B-cell chronic lymphocytic 
      leukemia patients: association of trisomy 12 and del(13q14).
PG  - 154-60
AB  - We have evaluated genomic aberrations by conventional cytogenetics and 
      fluorescence in situ hybridization (FISH) analysis in a series of 57 Argentinean 
      B-cell chronic lymphocytic leukemia (B-CLL) patients. The studies were performed 
      on stimulated peripheral blood lymphocytes. FISH analysis for trisomy 12, 13q14 
      deletion, and monosomy of TP53 (also known as p53) was performed according to 
      standard protocols. Our results showed 46.3% of patients with clonal chromosomal 
      alterations by conventional cytogenetics and 80.7% by FISH. Trisomy 12 was found 
      in 21.9% of patients by G-banding analysis and in 35% by FISH studies. Allelic 
      loss of 13q14 was observed in 63.2% patients, most of them showing D13S319 and 
      D13S25 deletion; 11% of patients showed TP53 monosomy. Coexistence of trisomy 12 
      and 13q14 deletion was found in 17.5% of patients. In this group, deletion 13q14 
      was the prevalent clone, with percentages 25-35% higher than those observed for 
      trisomy 12, suggesting clonal evolution. The coexistence of trisomy 12 with 
      deletion 13q14 was observed in a higher frequency than reported in the 
      literature. A probable adverse prognosis is suggested for this group of patients, 
      likely related to clonal evolution.
FAU - Chena, Christian
AU  - Chena C
AD  - Departamento de Genetica, Instituto de Investigaciones Hematologicas, Academia 
      Nacional de Medicina, Buenos Aires, Argentina. cpchena@hematologia.anm.edu.ar
FAU - Arrossagaray, Guillermo
AU  - Arrossagaray G
FAU - Scolnik, Mariano
AU  - Scolnik M
FAU - Palacios, Maria F
AU  - Palacios MF
FAU - Slavutsky, Irma
AU  - Slavutsky I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - Argentina
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 12
MH  - *Chromosomes, Human, Pair 13
MH  - Cytogenetic Analysis
MH  - Female
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Trisomy
EDAT- 2003/10/14 05:00
MHDA- 2003/11/05 05:00
CRDT- 2003/10/14 05:00
PHST- 2003/10/14 05:00 [pubmed]
PHST- 2003/11/05 05:00 [medline]
PHST- 2003/10/14 05:00 [entrez]
AID - S0165460803001365 [pii]
AID - 10.1016/s0165-4608(03)00136-5 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2003 Oct 15;146(2):154-60. doi: 
      10.1016/s0165-4608(03)00136-5.