PMID- 14555401
OWN - NLM
STAT- MEDLINE
DCOM- 20031118
LR  - 20191108
IS  - 1528-7394 (Print)
IS  - 0098-4108 (Linking)
VI  - 66
IP  - 21
DP  - 2003 Nov 14
TI  - Increased expression of glial fibrillary acidic protein in cerebellum and 
      hippocampus: differential effects on neonatal brain regional acetylcholinesterase 
      following maternal exposure to combined chlorpyrifos and nicotine.
PG  - 2047-66
AB  - Cigarette smoking and environmental exposure to chlorpyrifos during pregnancy 
      could lead to developmental toxicity in the offspring. In the present study, 
      pregnant female Sprague-Dawley rats (300-350 g) were treated daily with nicotine 
      (1 mg/kg, sc) or chlorpyrifos (0.1 mg/kg, dermal) or a combination of nicotine 
      and chlorpyrifos from gestational days (GD) 4-20. Control animals were treated 
      with saline and ethanol. Male offspring from the mothers treated with nicotine 
      alone gained significantly less weight on postnatal day (PND) 30 as compared to 
      control. On PND 7, there was a significant increase in brain acetylcholinesterase 
      (AChE) activity in pups from nicotine- and chlorpyrifos-treated dams, whereas 
      plasma butyrylcholinesterase (BChE) activity was significantly elevated in pups 
      of mothers treated with either chlorpyrifos alone or pesticide combined with 
      nicotine. On PND 30 there was a significant increase in AChE activity in 
      brainstem and cerebellum in all treated male pups. In female pups on PND 30 there 
      was a significant rise in AChE activity in brainstem of chlorpyrifos alone and in 
      cerebellum of the combination nicotine and chlorpyrifos group. Histopathological 
      evaluation demonstrated an increased neuronal cell death in the cerebellum 
      granular cell layer of female offspring from nicotine or combined nicotine with 
      chlorpyrifos group. A rise in glial fibrillary acidic protein (GFAP) 
      immunostaining was observed in the CA1 subfield of hippocampus and cerebellum on 
      PND 30 in female and male offspring of mothers treated with either nicotine or 
      nicotine in combination with chlorpyrifos, but to a lesser extent in males. Data 
      suggest that maternal exposure to nicotine and chlorpyrifos, alone or in 
      combination, produces differential alterations in brain regional AChE activity 
      and expression of GFAP in cerebellum and hippocampus in offspring on PND 30.
FAU - Abdel-Rahman, Ali
AU  - Abdel-Rahman A
AD  - Department of Pharmacology and Cancer Biology, Duke University Medical Center, 
      Durham, North Carolina 27710, USA.
FAU - Dechkovskaia, Anjelika
AU  - Dechkovskaia A
FAU - Mehta-Simmons, Heena
AU  - Mehta-Simmons H
FAU - Guan, Xiangrong
AU  - Guan X
FAU - Khan, Wasiuddin
AU  - Khan W
FAU - Abou-Donia, Mohamed
AU  - Abou-Donia M
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Toxicol Environ Health A
JT  - Journal of toxicology and environmental health. Part A
JID - 100960995
RN  - 0 (Ganglionic Stimulants)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Insecticides)
RN  - 6M3C89ZY6R (Nicotine)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
RN  - JCS58I644W (Chlorpyrifos)
SB  - IM
MH  - Acetylcholinesterase/analysis/*biosynthesis
MH  - Administration, Topical
MH  - Animals
MH  - Cell Death
MH  - Cerebellum/pathology/*physiology
MH  - Chlorpyrifos/administration & dosage/*toxicity
MH  - Drug Interactions
MH  - Female
MH  - Ganglionic Stimulants/administration & dosage/*toxicity
MH  - Glial Fibrillary Acidic Protein/*biosynthesis
MH  - Hippocampus/pathology/*physiology
MH  - Injections, Subcutaneous
MH  - Insecticides/administration & dosage/*toxicity
MH  - Male
MH  - Neurons
MH  - Nicotine/administration & dosage/*toxicity
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2003/10/14 05:00
MHDA- 2003/12/03 05:00
CRDT- 2003/10/14 05:00
PHST- 2003/10/14 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/10/14 05:00 [entrez]
AID - R3D93LBRL0EMCVF8 [pii]
AID - 10.1080/713853982 [doi]
PST - ppublish
SO  - J Toxicol Environ Health A. 2003 Nov 14;66(21):2047-66. doi: 10.1080/713853982.