PMID- 14555468
OWN - NLM
STAT- MEDLINE
DCOM- 20040713
LR  - 20210526
IS  - 1535-9778 (Print)
IS  - 1535-9786 (Electronic)
IS  - 1535-9786 (Linking)
VI  - 2
IP  - 5
DP  - 2003 Oct
TI  - Chromosome pairing does not contribute to nuclear architecture in vegetative 
      yeast cells.
PG  - 856-66
AB  - There are several reports of a closer-than-random colocalization of homologous 
      chromosomes in the vegetative nuclei of diploid budding yeast. Here, we studied 
      by fluorescence in situ hybridization (FISH) the nuclear distribution of 
      chromosomes and found a slight tendency toward closer proximity between 
      homologous (allelic) loci than between any nonhomologous chromosomal regions. We 
      show that most of this preferential association is not due to vegetative (also 
      known as somatic) pairing but is caused by the polar orientation of interphase 
      chromosomes (Rabl orientation). We quantified the occasional loss of detectable 
      fluorescence signals that is inherent to the FISH method. Signal loss leads to 
      the occurrence of a single signal that may be misinterpreted as the close 
      association of two homologous chromosomal sites. The nuclear distribution of 
      homologous loci, when corrected for the influence of nuclear architecture and 
      methodological faults, was not different or was only marginally different from a 
      random relative positioning as predicted by computer simulation. We discuss here 
      several possibilities for the residual homologous proximity that do not invoke 
      homology-dependent vegetative pairing, and we conclude that, in diploid budding 
      yeast, constitutive vegetative pairing is a negligible factor for the 
      organization of the interphase nucleus.
FAU - Lorenz, Alexander
AU  - Lorenz A
AD  - Institute of Botany, University of Vienna, Vienna, Austria.
FAU - Fuchs, Jorg
AU  - Fuchs J
FAU - Burger, Reinhard
AU  - Burger R
FAU - Loidl, Josef
AU  - Loidl J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Eukaryot Cell
JT  - Eukaryotic cell
JID - 101130731
SB  - IM
MH  - Cell Nucleus/*physiology
MH  - Centromere/physiology
MH  - Chromosome Pairing/*physiology
MH  - Computer Simulation
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/physiology
MH  - Meiosis/physiology
MH  - Microscopy, Fluorescence
MH  - Nucleolus Organizer Region/physiology
MH  - Saccharomyces cerevisiae/physiology
MH  - Yeasts/*physiology
PMC - PMC219365
EDAT- 2003/10/14 05:00
MHDA- 2004/07/14 05:00
PMCR- 2003/10/01
CRDT- 2003/10/14 05:00
PHST- 2003/10/14 05:00 [pubmed]
PHST- 2004/07/14 05:00 [medline]
PHST- 2003/10/14 05:00 [entrez]
PHST- 2003/10/01 00:00 [pmc-release]
AID - 0085 [pii]
AID - 10.1128/EC.2.5.856-866.2003 [doi]
PST - ppublish
SO  - Eukaryot Cell. 2003 Oct;2(5):856-66. doi: 10.1128/EC.2.5.856-866.2003.