PMID- 14556801
OWN - NLM
STAT- MEDLINE
DCOM- 20031124
LR  - 20190707
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 80
IP  - 4
DP  - 2003 Oct
TI  - Preimplantation aneuploidy screening using comparative genomic hybridization or 
      fluorescence in situ hybridization of embryos from patients with recurrent 
      implantation failure.
PG  - 860-8
AB  - OBJECTIVE: To select chromosomally euploid embryos for transfer by analyzing 
      single biopsied blastomeres using either fluorescence in situ hybridization 
      (FISH) for chromosomes 13, 16, 18, 21, and 22 or comparative genomic 
      hybridization (CGH), which provides a full karyotype. DESIGN: Prospective 
      observational study. SETTING: A large IVF unit and the research laboratory of a 
      hospital clinical genetics unit. PATIENT(S): Twenty patients with recurrent 
      implantation failure. INTERVENTION(S): Ovarian stimulation and IVF by 
      intracytoplasmic sperm injection (ICSI), embryo biopsy, and embryo transfer. MAIN 
      OUTCOME MEASURE(S): Chromosome normality of biopsied blastomeres and implantation 
      and clinical pregnancy rates. RESULT(S): Comparative genomic hybridization was 
      able to identify many chromosomal abnormalities that would have been missed if 
      those cells had been analyzed by FISH. The clinical pregnancy rate per transfer 
      and implantation rate was 11% and 7% for embryos analyzed by FISH and 21% and 15% 
      for embryos analyzed by CGH. CONCLUSION(S): Comparative genomic hybridization is 
      more effective than FISH for identifying chromosomally normal embryos, which may 
      result in a higher clinical pregnancy rate and implantation rate after embryo 
      transfer.
FAU - Wilton, Leeanda
AU  - Wilton L
AD  - Melbourne IVF, Freemason's Hospital Medical Centre, East Melbourne, Victoria, 
      Australia. lwilton@mivf.com.au
FAU - Voullaire, Lucille
AU  - Voullaire L
FAU - Sargeant, Peta
AU  - Sargeant P
FAU - Williamson, Robert
AU  - Williamson R
FAU - McBain, John
AU  - McBain J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
CIN - Fertil Steril. 2003 Oct;80(4):869-70; discussion 875. PMID: 14556802
CIN - Fertil Steril. 2003 Oct;80(4):871-2; discussion 875. PMID: 14556803
CIN - Fertil Steril. 2003 Oct;80(4):873-4; discussion 875. PMID: 14556804
MH  - Adult
MH  - *Aneuploidy
MH  - Cryopreservation
MH  - *Embryo Implantation
MH  - *Embryo Transfer
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping/methods
MH  - *Nucleic Acid Hybridization
MH  - Pregnancy
MH  - Pregnancy Rate
MH  - *Preimplantation Diagnosis/methods
MH  - Prospective Studies
MH  - Recurrence
MH  - Treatment Failure
EDAT- 2003/10/15 05:00
MHDA- 2003/12/03 05:00
CRDT- 2003/10/15 05:00
PHST- 2003/10/15 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/10/15 05:00 [entrez]
AID - S0015028203011622 [pii]
AID - 10.1016/s0015-0282(03)01162-2 [doi]
PST - ppublish
SO  - Fertil Steril. 2003 Oct;80(4):860-8. doi: 10.1016/s0015-0282(03)01162-2.