PMID- 14557544
OWN - NLM
STAT- MEDLINE
DCOM- 20040105
LR  - 20181113
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 100
IP  - 22
DP  - 2003 Oct 28
TI  - Brain-derived neurotrophic factor-dependent unmasking of "silent" synapses in the 
      developing mouse barrel cortex.
PG  - 13069-74
AB  - Brain-derived neurotrophic factor (BDNF) is a critical modulator of central 
      synaptic functions such as long-term potentiation in the hippocampal and visual 
      cortex. Little is known, however, about its role in the development of excitatory 
      glutamatergic synapses in vivo. We investigated the development of 
      N-methyl-D-aspartate (NMDA) receptor (NMDAR)-only synapses (silent synapses) and 
      found that silent synapses were prominent in acute thalamocortical brain slices 
      from BDNF knockout mice even after the critical period. These synapses could be 
      partially converted to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid 
      receptor (AMPAR)-containing ones by adding back BDNF alone to the slice or fully 
      converted to together with electric stimulation without affecting NMDAR 
      transmission. Electric stimulation alone was ineffective under the BDNF knockout 
      background. Postsynaptically applied TrkB kinase inhibitor or calcium-chelating 
      reagent blocked this conversion. Furthermore, the AMPAR C-terminal peptides 
      essential for interaction with PDZ proteins postsynaptically prevented the 
      unmasking of silent synapses. These results suggest that endogenous BDNF and 
      neuronal activity synergistically activate AMPAR trafficking into synaptic sites.
FAU - Itami, Chiaki
AU  - Itami C
AD  - Division of Biochemistry and Cellular Biology, National Institute of 
      Neuroscience, Tokyo 187-8502, Japan.
FAU - Kimura, Fumitaka
AU  - Kimura F
FAU - Kohno, Tomoko
AU  - Kohno T
FAU - Matsuoka, Masato
AU  - Matsuoka M
FAU - Ichikawa, Masumi
AU  - Ichikawa M
FAU - Tsumoto, Tadaharu
AU  - Tsumoto T
FAU - Nakamura, Shun
AU  - Nakamura S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031013
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Receptors, AMPA)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/deficiency/genetics/pharmacology/*physiology
MH  - Calcium/physiology
MH  - Hippocampus/growth & development/*physiology
MH  - Mice
MH  - Mice, Knockout
MH  - Neurons/physiology
MH  - Receptors, AMPA/drug effects/physiology
MH  - Somatosensory Cortex/growth & development/*physiology
MH  - Synapses/*physiology
MH  - Visual Cortex/growth & development/*physiology
PMC - PMC240746
EDAT- 2003/10/15 05:00
MHDA- 2004/01/06 05:00
PMCR- 2004/04/28
CRDT- 2003/10/15 05:00
PHST- 2003/10/15 05:00 [pubmed]
PHST- 2004/01/06 05:00 [medline]
PHST- 2003/10/15 05:00 [entrez]
PHST- 2004/04/28 00:00 [pmc-release]
AID - 2131948100 [pii]
AID - 10013069 [pii]
AID - 10.1073/pnas.2131948100 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):13069-74. doi: 
      10.1073/pnas.2131948100. Epub 2003 Oct 13.