PMID- 14559079
OWN - NLM
STAT- MEDLINE
DCOM- 20031215
LR  - 20201208
IS  - 0041-0101 (Print)
IS  - 0041-0101 (Linking)
VI  - 42
IP  - 3
DP  - 2003 Sep
TI  - Nephrotoxic effects of chronic administration of microcystins -LR and -YR.
PG  - 281-8
AB  - Acute intoxication with MC-LR induces cytoskeletal alterations, apoptosis and 
      necrosis of hepatocytes resulting in intrahepatic hemorrhage. Preliminary results 
      have shown that chronic treatment of rats with intraperitoneal injections of 
      sublethal doses of microcystins MC-LR and MC-YR could induce not only liver, but 
      also kidney injuries. We aimed to investigate whether the induction of the 
      cytoskeletal changes, apoptosis and necrosis could be the mechanisms involved in 
      the injury of kidney cells in the chronic model of microcystin intoxication. 
      Experimental rats were receiving intraperitoneal injections of MC-LR (10 
      microg/kg) or MC-YR (10 microg/kg) every second day for 8 months, while control 
      rats were receiving only the vehicle. The histopathological investigation 
      revealed collapsed glomeruli with thickened basement membranes and dilated tubuli 
      filled with eosinophilic casts. Rhodamine-phalloidin labeling showed cytoplasmic 
      aggregation and accumulation of fibrilar actin filaments within the epithelial 
      tubular cells. Terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) showed increased number of TUNEL-positive cells in the kidney 
      cortex and medulla. The pathological changes induced by MC-LR appeared more 
      severe than those induced by MC-YR. The results support the view that at the 
      cellular level, the mechanisms that underly the chronic nephrotoxicity are 
      similar to the mechanisms of the acute hepatotoxicity of microcystins.
FAU - Milutinovic, Aleksandra
AU  - Milutinovic A
AD  - Institute of Pathophysiology, Medical Faculty of Ljubljana, University of 
      Ljubljana, Zaloska 4, 1000 Ljubljana, Slovenia.
FAU - Zivin, Marko
AU  - Zivin M
FAU - Zorc-Pleskovic, Ruda
AU  - Zorc-Pleskovic R
FAU - Sedmak, Bojan
AU  - Sedmak B
FAU - Suput, Dusan
AU  - Suput D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Toxicon
JT  - Toxicon : official journal of the International Society on Toxinology
JID - 1307333
RN  - 0 (Bacterial Toxins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Marine Toxins)
RN  - 0 (Microcystins)
RN  - 0 (Peptides, Cyclic)
RN  - 101064-48-6 (microcystin YR)
RN  - EQ8332842Y (cyanoginosin LR)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Bacterial Toxins/*toxicity
MH  - Cytoskeletal Proteins/drug effects
MH  - Cytoskeleton/*drug effects/pathology
MH  - Enzyme Inhibitors/toxicity
MH  - Kidney/*drug effects/pathology
MH  - Male
MH  - Marine Toxins
MH  - Microcystins
MH  - Necrosis
MH  - Peptides, Cyclic/*toxicity
MH  - Rats
MH  - Rats, Wistar
MH  - Renal Circulation/drug effects
MH  - Toxicity Tests, Chronic
EDAT- 2003/10/16 05:00
MHDA- 2003/12/16 05:00
CRDT- 2003/10/16 05:00
PHST- 2003/10/16 05:00 [pubmed]
PHST- 2003/12/16 05:00 [medline]
PHST- 2003/10/16 05:00 [entrez]
AID - S0041010103001430 [pii]
AID - 10.1016/s0041-0101(03)00143-0 [doi]
PST - ppublish
SO  - Toxicon. 2003 Sep;42(3):281-8. doi: 10.1016/s0041-0101(03)00143-0.