PMID- 14559170
OWN - NLM
STAT- MEDLINE
DCOM- 20040107
LR  - 20210119
IS  - 0378-1135 (Print)
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 96
IP  - 3
DP  - 2003 Oct 30
TI  - Lymphoid tissue tropism of porcine reproductive and respiratory syndrome virus 
      replication during persistent infection of pigs originally exposed to virus in 
      utero.
PG  - 219-35
AB  - The ability of porcine reproductive and respiratory syndrome virus (PRRSV) to 
      establish a persistent infection is the principal contributing factor to the 
      world-wide spread of the disease. Several studies have documented the course of 
      viral infection in postnatally infected pigs; however, very little is known 
      regarding sites of virus replication during persistent infection of pigs exposed 
      to PRRSV in utero. In this study, virus replication and PRRSV-specific antibody 
      were followed for several hundred days in a group of pigs derived from three sows 
      infected at 90 days of gestation with PRRSV isolate VR-2332. Eighty-four percent 
      of pigs were born viremic with a mortality of 54% within 21 days after birth. At 
      approximately 60 days sera from pigs were negative for virus by virus isolation. 
      Analysis of virus replication in the tissues of pigs randomly sacrificed between 
      63 and 132 days showed no evidence of virus in lung and other non-lymphoid 
      organs. However, virus was easily recovered from tonsil and lymph nodes and in 
      situ hybridization identified these tissues as sites of virus replication. Even 
      though replication was at a low level, virus was easily transmitted to sentinel 
      pigs. By 260 days pigs became seronegative and did not transmit virus to sentinel 
      pigs. Sacrifice of remaining pigs after 300 days showed no evidence of virus in 
      blood and tissues. This study shows that congenital PRRSV-infected pigs can 
      support virus replication for an extended period during which virus replication 
      is primarily restricted to tonsil and lymph nodes.
FAU - Rowland, Raymond R R
AU  - Rowland RR
AD  - Department of Diagnostic Medicine and Pathobiology, 1800 Denison Avenue, Kansas 
      State University, Manhattan, KS 66506, USA. browland@vet.ksu.edu
FAU - Lawson, Steven
AU  - Lawson S
FAU - Rossow, Kurt
AU  - Rossow K
FAU - Benfield, David A
AU  - Benfield DA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antibodies, Viral/blood
MH  - Carrier State/veterinary/virology
MH  - Female
MH  - In Situ Hybridization/veterinary
MH  - Lymphoid Tissue/*virology
MH  - Neutralization Tests/veterinary
MH  - Organ Specificity
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*growth & development
MH  - Pregnancy
MH  - RNA, Viral/chemistry/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Swine
MH  - Umbilical Cord/virology
MH  - Virus Replication/*physiology
PMC - PMC7172578
EDAT- 2003/10/16 05:00
MHDA- 2004/01/08 05:00
PMCR- 2003/09/25
CRDT- 2003/10/16 05:00
PHST- 2003/10/16 05:00 [pubmed]
PHST- 2004/01/08 05:00 [medline]
PHST- 2003/10/16 05:00 [entrez]
PHST- 2003/09/25 00:00 [pmc-release]
AID - S0378113503002463 [pii]
AID - 10.1016/j.vetmic.2003.07.006 [doi]
PST - ppublish
SO  - Vet Microbiol. 2003 Oct 30;96(3):219-35. doi: 10.1016/j.vetmic.2003.07.006.