PMID- 14563966
OWN - NLM
STAT- MEDLINE
DCOM- 20031125
LR  - 20161122
IS  - 1524-4628 (Electronic)
IS  - 0039-2499 (Linking)
VI  - 34
IP  - 11
DP  - 2003 Nov
TI  - Intraventricular infusion of TrkB-Fc fusion protein promotes ischemia-induced 
      neurogenesis in adult rat dentate gyrus.
PG  - 2710-5
AB  - BACKGROUND AND PURPOSE: We have previously shown that delivery of brain-derived 
      neurotrophic factor (BDNF) through direct intrahippocampal gene transduction with 
      a viral vector suppresses the formation of new dentate granule cells triggered by 
      global forebrain ischemia. Here, we investigated whether inhibition of endogenous 
      BDNF alters ischemia-induced neurogenesis in the dentate gyrus. METHODS: Rats 
      were subjected to 30 minutes of global forebrain ischemia and then received 
      intraventricular infusion of either the BDNF scavenger, TrkB-Fc fusion protein, 
      or control Hu-Fc for 2 weeks. In parallel, all animals were injected 
      intraperitoneally with the mitosis marker 
      5-bromo-2'-deoxyuridine-5'-monophosphate (BrdU). Animals were killed at 2 or 6 
      weeks after the ischemic insult, and neurogenesis was then assessed 
      immunocytochemically with epifluorescence or confocal microscopy. RESULTS: 
      Infusion of TrkB-Fc fusion protein gave rise to elevated numbers of 
      ischemia-generated new neurons, double-labeled with BrdU and the early neuronal 
      marker Hu or the mature neuronal marker NeuN, in the dentate subgranular zone and 
      granule cell layer at 2 and 6 weeks after the insult. CONCLUSIONS: Our findings 
      provide evidence that endogenous BDNF counteracts neuronal differentiation, but 
      not cell proliferation or survival, in ischemia-induced dentate gyrus 
      neurogenesis.
FAU - Gustafsson, Elin
AU  - Gustafsson E
AD  - Section of Restorative Neurology, Wallenberg Neuroscience Center, University 
      Hospital, Lund, Sweden.
FAU - Lindvall, Olle
AU  - Lindvall O
FAU - Kokaia, Zaal
AU  - Kokaia Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031016
PL  - United States
TA  - Stroke
JT  - Stroke
JID - 0235266
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Immunoglobulin Fc Fragments)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation/biosynthesis
MH  - Brain Ischemia/*physiopathology
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Bromodeoxyuridine
MH  - Cell Differentiation/drug effects
MH  - Cell Division/drug effects
MH  - Dentate Gyrus/cytology/*drug effects/physiopathology
MH  - Disease Models, Animal
MH  - Immunoglobulin Fc Fragments/*genetics
MH  - Injections, Intraventricular
MH  - Male
MH  - Neurons/cytology/*drug effects/metabolism
MH  - Prosencephalon/blood supply/physiopathology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/*genetics
MH  - Recombinant Fusion Proteins/administration & dosage/*pharmacology
EDAT- 2003/10/18 05:00
MHDA- 2003/12/03 05:00
CRDT- 2003/10/18 05:00
PHST- 2003/10/18 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/10/18 05:00 [entrez]
AID - 01.STR.0000096025.35225.36 [pii]
AID - 10.1161/01.STR.0000096025.35225.36 [doi]
PST - ppublish
SO  - Stroke. 2003 Nov;34(11):2710-5. doi: 10.1161/01.STR.0000096025.35225.36. Epub 
      2003 Oct 16.