PMID- 14568894
OWN - NLM
STAT- MEDLINE
DCOM- 20031124
LR  - 20210507
IS  - 1524-4539 (Electronic)
IS  - 0009-7322 (Linking)
VI  - 108
IP  - 17
DP  - 2003 Oct 28
TI  - Transdifferentiation of human peripheral blood CD34+-enriched cell population 
      into cardiomyocytes, endothelial cells, and smooth muscle cells in vivo.
PG  - 2070-3
AB  - BACKGROUND: Adult human peripheral blood cells have been shown to differentiate 
      into mature cells of nonhematopoietic tissues, such as hepatocytes and epithelial 
      cells of the skin and gastrointestinal track. We investigated whether these cells 
      could also transdifferentiate into human cardiomyocytes, mature endothelial 
      cells, and smooth muscle cells in vivo. METHODS AND RESULTS: Myocardial 
      infarction was created in SCID mice by occluding the left anterior descending 
      coronary artery, after which adult peripheral blood CD34+ cells were injected 
      into the tail vein. Hearts were harvested 2 months after injection and stained 
      for human leukocyte antigen (HLA) and markers for cardiomyocytes, endothelial 
      cells, and smooth muscle cells. Cardiomyocytes, endothelial cells, and smooth 
      muscle cells that bear HLA were identified in the infarct and peri-infarct 
      regions of the mouse hearts. In a separate experiment, CD34+ cells were injected 
      intraventricularly into mice without experimental myocardial infarction. 
      HLA-positive myocytes and smooth muscle cells could only be identified in 1 of 
      these mice killed at different time points. CONCLUSIONS: Adult peripheral blood 
      CD34+ cells can transdifferentiate into cardiomyocytes, mature endothelial cells, 
      and smooth muscle cells in vivo. However, transdifferentiation is augmented 
      significantly by local tissue injury. The use of peripheral blood CD34+ cells for 
      cell-based therapy should greatly simplify the procurement of cells for the 
      regeneration of damaged myocardium.
FAU - Yeh, Edward T H
AU  - Yeh ET
AD  - Department of Cardiology, The University of Texas-M.D. Anderson Cancer Center, 
      Houston, Tex, USA. etyeh@mdanderson.org
FAU - Zhang, Sui
AU  - Zhang S
FAU - Wu, Henry D
AU  - Wu HD
FAU - Korbling, Martin
AU  - Korbling M
FAU - Willerson, James T
AU  - Willerson JT
FAU - Estrov, Zeev
AU  - Estrov Z
LA  - eng
PT  - Journal Article
DEP - 20031020
PL  - United States
TA  - Circulation
JT  - Circulation
JID - 0147763
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Animals
MH  - Antigens, CD34/*biosynthesis
MH  - Antigens, Differentiation/biosynthesis
MH  - Cell Differentiation/*physiology
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Endothelium, Vascular/cytology/*physiology
MH  - Flow Cytometry
MH  - Graft Survival
MH  - HLA Antigens/biosynthesis/immunology
MH  - Humans
MH  - Mice
MH  - Mice, SCID
MH  - Muscle, Smooth, Vascular/cytology/*physiology
MH  - Myocardial Infarction/pathology/*therapy
MH  - Myocytes, Cardiac/cytology/*physiology
MH  - Stem Cell Transplantation
MH  - Stem Cells/cytology/*metabolism
EDAT- 2003/10/22 05:00
MHDA- 2003/12/03 05:00
CRDT- 2003/10/22 05:00
PHST- 2003/10/22 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/10/22 05:00 [entrez]
AID - 01.CIR.0000099501.52718.70 [pii]
AID - 10.1161/01.CIR.0000099501.52718.70 [doi]
PST - ppublish
SO  - Circulation. 2003 Oct 28;108(17):2070-3. doi: 10.1161/01.CIR.0000099501.52718.70. 
      Epub 2003 Oct 20.