PMID- 14570621
OWN - NLM
STAT- MEDLINE
DCOM- 20040227
LR  - 20041117
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 116
IP  - 10
DP  - 2003 Oct
TI  - Increased apoptosis and decreased density of medial smooth muscle cells in human 
      abdominal aortic aneurysms.
PG  - 1549-52
AB  - OBJECTIVE: To determine the increase of apoptosis and the decrease of smooth 
      muscle cells (SMCs) density in human abdominal aortic aneurysms (AAA). METHODS: 
      In situ terminal transferase-mediated dUTP nick end labeling (TUNEL) was employed 
      to detect apoptosis of SMCs in patients with AAA (n = 25) and normal abdominal 
      aortae (n = 10). Positive cells were identified by specific cell marker in 
      combination with immunohistochemistry. Meanwhile SMC counting was performed by 
      anti-alpha-actin immunohistostaining to compare the SMC density. RESULTS: TUNEL 
      staining revealed that there was significantly increased apoptosis in AAAs 
      (average 8.6%) compared with normal abdominal aortae (average 0.95%, P < 0.01). 
      Double staining showed that most of these cells were SMCs. Counting of 
      alpha-actin positive SMCs revealed that medial SMC density of AAAs (37.5 +/- 7.6 
      SMCs/HPF) was reduced by 79.1% in comparison with that of normal abdominal aortae 
      (179.2 +/- 16.1 SMCs/HPF, P < 0.01). CONCLUSIONS: Significantly increased SMCs of 
      AAA bear apoptotic markers initiating cell death. Elevated apoptosis may result 
      in a decreased density of SMCs in AAA, which may profoundly influence the 
      development of AAA.
FAU - Zhang, Jian
AU  - Zhang J
AD  - Department of Vascular Surgery, the First Affiliated Hospital, China Medical 
      University, Shenyang 110001, China. jianzhang_cmu@yahoo.com.cn
FAU - Schmidt, Jan
AU  - Schmidt J
FAU - Ryschich, Eduard
AU  - Ryschich E
FAU - Schumacher, Hardy
AU  - Schumacher H
FAU - Allenberg, Jens R
AU  - Allenberg JR
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Aortic Aneurysm, Abdominal/*pathology
MH  - Apoptosis/*physiology
MH  - Cell Count
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Middle Aged
MH  - Muscle, Smooth, Vascular/*pathology
EDAT- 2003/10/23 05:00
MHDA- 2004/02/28 05:00
CRDT- 2003/10/23 05:00
PHST- 2003/10/23 05:00 [pubmed]
PHST- 2004/02/28 05:00 [medline]
PHST- 2003/10/23 05:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2003 Oct;116(10):1549-52.