PMID- 14570981
OWN - NLM
STAT- MEDLINE
DCOM- 20040126
LR  - 20071114
IS  - 1531-2267 (Electronic)
IS  - 1094-8341 (Linking)
VI  - 16
IP  - 1
DP  - 2003 Dec 16
TI  - Serial analysis of gene expression in mouse kidney following angiotensin II 
      administration.
PG  - 90-8
AB  - As a new line of inquiry into the molecular mechanisms underlying 
      pathophysiological processes associated with angiotensin (ANG II)-dependent 
      hypertension, we applied the method of serial analysis of gene expression (SAGE) 
      to examine genome-wide transcription changes in the kidneys of mice that 
      developed hypertension in response to chronic ANG II administration. Mice were 
      infused subcutaneously via osmotic minipumps with ANG II for 7 days, and systolic 
      blood pressure was measured by tail-cuff plethysmography. Subsequently, mice were 
      euthanized, and the total RNA isolated from the kidneys was used to construct 
      SAGE libraries. Comparison of 11,447 SAGE tags from the hypertensive kidneys, 
      representing 5,740 unique transcripts, and 11,273 tags from the control kidneys, 
      corresponding to 5,619 different transcripts, identified genes that are 
      significantly (P < 0.05) down- or upregulated in the hypertensive kidney. Our 
      assessment of the genome-wide influence of ANG II resulted in the detection of 
      several novel genes and in a recognition of potential new roles for the 
      previously characterized genes, thus providing new probes with which to further 
      explore the ANG II effects in normal and disease states.
FAU - Schwartz, Faina
AU  - Schwartz F
AD  - Department of Medicine, Hypertension Section, Boston University School of 
      Medicine, Boston, Massachusetts 02118, USA. fschw@bu.edu
FAU - Duka, Arvi
AU  - Duka A
FAU - Triantafyllidi, Elena
AU  - Triantafyllidi E
FAU - Johns, Conrado
AU  - Johns C
FAU - Duka, Irena
AU  - Duka I
FAU - Cui, Jing
AU  - Cui J
FAU - Gavras, Haralambos
AU  - Gavras H
LA  - eng
GR  - P50-HL-55001/HL/NHLBI NIH HHS/United States
GR  - U01-HL-66617/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20031216
PL  - United States
TA  - Physiol Genomics
JT  - Physiological genomics
JID - 9815683
RN  - 11128-99-7 (Angiotensin II)
SB  - IM
MH  - Angiotensin II/administration & dosage/*pharmacology
MH  - Animals
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation/*drug effects
MH  - Gene Library
MH  - Genome
MH  - *Genomics
MH  - Hypertension/*genetics
MH  - Kidney/*drug effects/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Reproducibility of Results
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2003/10/23 05:00
MHDA- 2004/01/27 05:00
CRDT- 2003/10/23 05:00
PHST- 2003/10/23 05:00 [pubmed]
PHST- 2004/01/27 05:00 [medline]
PHST- 2003/10/23 05:00 [entrez]
AID - 00108.2003 [pii]
AID - 10.1152/physiolgenomics.00108.2003 [doi]
PST - epublish
SO  - Physiol Genomics. 2003 Dec 16;16(1):90-8. doi: 
      10.1152/physiolgenomics.00108.2003.