PMID- 14571137
OWN - NLM
STAT- MEDLINE
DCOM- 20040315
LR  - 20171101
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 101
IP  - 1
DP  - 2003
TI  - Cloning and genomic organization of the TTL gene on mouse chromosome 2 and human 
      chromosome 2q13.
PG  - 47-53
AB  - Tubulin tyrosine ligase (TTL) is a cytosolic enzyme involved in the 
      posttranslational modification of tubulin. In the assembled form microtubules are 
      detyrosinated over time at the C-terminus of alpha-tubulin. After microtubular 
      disassembly TTL restores tyrosine residues back to the detyrosinated tubulin 
      leading to a cycle of detyrosination/tyrosination. Here we report the isolation 
      of the human and mouse TTL cDNA. In comparison with other known TTL sequences, 
      namely bovine, rat and porcine, we found that only porcine TTL deviates in length 
      by having an insertion of two glutamate residues. In mouse and human TTL the 
      genomic coding sequence is composed of seven exons with normal intron/exon 
      boundaries. Using fluorescence in situ hybridization (FISH), we mapped the murine 
      TTL gene to mouse chromosome 2 (MMU2). Human TTL has been located to chromosome 
      2q13 (HSA2q13). In addition, we found frequently truncated PCR products of hTTL 
      transcripts with aberrant splicing in tumors.
CI  - Copyright 2003 S. Karger AG, Basel
FAU - Erck, C
AU  - Erck C
AD  - Department of Cell Biology, German Research Center of Biotechnology, 
      Braunschweig, Germany. cer@gbf.de
FAU - MacLeod, R A F
AU  - MacLeod RA
FAU - Wehland, J
AU  - Wehland J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA, Complementary)
RN  - 9007-49-2 (DNA)
RN  - EC 6.3.2.- (Peptide Synthases)
RN  - EC 6.3.2.- (tyrosyltubulin ligase)
SB  - IM
MH  - 5' Flanking Region/genetics
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line, Tumor
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 2/*genetics
MH  - Chromosomes, Mammalian/*genetics
MH  - Cloning, Molecular
MH  - DNA/chemistry/genetics
MH  - DNA, Complementary/chemistry/genetics
MH  - Exons
MH  - Genes/genetics
MH  - HeLa Cells
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Introns
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Molecular Sequence Data
MH  - NIH 3T3 Cells
MH  - Peptide Synthases/*genetics
MH  - Phylogeny
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
EDAT- 2003/10/23 05:00
MHDA- 2004/03/17 05:00
CRDT- 2003/10/23 05:00
PHST- 2002/12/11 00:00 [received]
PHST- 2003/04/09 00:00 [accepted]
PHST- 2003/10/23 05:00 [pubmed]
PHST- 2004/03/17 05:00 [medline]
PHST- 2003/10/23 05:00 [entrez]
AID - 73418 [pii]
AID - 10.1159/000073418 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2003;101(1):47-53. doi: 10.1159/000073418.