PMID- 14571141
OWN - NLM
STAT- MEDLINE
DCOM- 20040315
LR  - 20171101
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 101
IP  - 1
DP  - 2003
TI  - Replication banding and FISH analysis reveal the origin of the Hyla femoralis 
      karyotype and XY/XX sex chromosomes.
PG  - 80-3
AB  - The pine woods treefrog, Hyla femoralis, is unique among North American hylid 
      frogs in having a metacentric chromosome 6 and heteromorphic sex chromosomes of 
      the XY/XX type. The X chromosome is distinguished by having a nucleolar 
      organizing region (NOR) in the short arm. The Y chromosome does not possess an 
      NOR. Until the present study, it was not known if the NOR was not present on the 
      Y chromosome or inactive and therefore not detectable by conventional cytogenetic 
      methods like silver staining. Exclusive of its unique features the karyotype of 
      H. femoralis closely resembles those of North American frogs with karyotypes like 
      H. chrysoscelis. We used replication banding and fluorescence in situ 
      hybridization (FISH) with a DNA probe to the 18S + 28S ribosomal genes, which are 
      located at the NOR, to characterize the H. femoralis karyotype. Our analysis 
      revealed that the 18S + 28S ribosomal genes are not present on the Y chromosome, 
      and that the karyotype of H. femoralis was derived from an H. chrysoscelis-like 
      karyotype by relocation of the NOR to the X chromosome from chromosome 6 and 
      either a concurrent or subsequent pericentric inversion of chromosome 6.
CI  - Copyright 2003 S. Karger AG, Basel
FAU - Wiley, J E
AU  - Wiley JE
AD  - Department of Pediatrics, The Brody School of Medicine, East Carolina University, 
      Greenville, NC 27858, USA. wileyj@mail.ecu.edu
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - Animals
MH  - Anura/*genetics
MH  - Chromosome Banding/*methods
MH  - DNA Replication/genetics
MH  - Evolution, Molecular
MH  - Female
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Male
MH  - Nucleolus Organizer Region/genetics
MH  - Sex Chromosomes/*genetics
MH  - X Chromosome/genetics
MH  - Y Chromosome/genetics
EDAT- 2003/10/23 05:00
MHDA- 2004/03/17 05:00
CRDT- 2003/10/23 05:00
PHST- 2003/06/04 00:00 [received]
PHST- 2003/06/06 00:00 [accepted]
PHST- 2003/10/23 05:00 [pubmed]
PHST- 2004/03/17 05:00 [medline]
PHST- 2003/10/23 05:00 [entrez]
AID - 73422 [pii]
AID - 10.1159/000073422 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2003;101(1):80-3. doi: 10.1159/000073422.