PMID- 14571983
OWN - NLM
STAT- MEDLINE
DCOM- 20040719
LR  - 20191210
IS  - 0141-5492 (Print)
IS  - 0141-5492 (Linking)
VI  - 25
IP  - 18
DP  - 2003 Sep
TI  - Molecular detection and direct enumeration of methanogenic Archaea and 
      methanotrophic Bacteria in domestic solid waste landfill soils.
PG  - 1563-9
AB  - Methane oxidizing and producing activities of cover soil (10, 30 cm depth) and 
      burial waste (1, 3 m depth) were evaluated: top cover soil (10 cm) had the 
      highest methane oxidizing activity, while 1 m depth buried waste showed the 
      highest methane producing potential. All the sequences of the 1 m sample were 
      found to be closely related to 16S rDNAs of mainly hydrogenotrophic methanogens 
      known, such as genera Methanosarcina, Methanoculleus, and Methanobacterium. We 
      developed a modified fluorescence in situ hybridization (FISH) direct counting 
      method for landfill samples, resulting in the detection of approx. 1% of total 
      cells as archaeal cells (presumably methanogens). However, probe-positive cells 
      could not be found with probes for methanotrophs by the methods.
FAU - Chen, Ann-Cheng
AU  - Chen AC
AD  - Department of Environmental Systems Engineering, Nagaoka University of 
      Technology, Nagaoka, Niigata 940-2188, Japan. encheng@mail.chna.edu.tw
FAU - Ueda, Kumiko
AU  - Ueda K
FAU - Sekiguchi, Yuji
AU  - Sekiguchi Y
FAU - Ohashi, Akiyoshi
AU  - Ohashi A
FAU - Harada, Hideki
AU  - Harada H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Netherlands
TA  - Biotechnol Lett
JT  - Biotechnology letters
JID - 8008051
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Archaea/genetics/isolation & purification
MH  - Colony Count, Microbial/*methods
MH  - Gene Expression Profiling/methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Methanobacterium/*genetics/*isolation & purification
MH  - RNA, Ribosomal, 16S/*analysis/*genetics
MH  - Refuse Disposal/methods
MH  - Sewage/*microbiology
MH  - *Soil Microbiology
EDAT- 2003/10/24 05:00
MHDA- 2004/07/20 05:00
CRDT- 2003/10/24 05:00
PHST- 2003/10/24 05:00 [pubmed]
PHST- 2004/07/20 05:00 [medline]
PHST- 2003/10/24 05:00 [entrez]
AID - 10.1023/a:1025461915495 [doi]
PST - ppublish
SO  - Biotechnol Lett. 2003 Sep;25(18):1563-9. doi: 10.1023/a:1025461915495.