PMID- 14572341
OWN - NLM
STAT- MEDLINE
DCOM- 20050315
LR  - 20131121
IS  - 1007-3418 (Print)
IS  - 1007-3418 (Linking)
VI  - 11
IP  - 10
DP  - 2003 Oct
TI  - [Detection and analysis of the new kind of G743C and G743A point mutation of HBV 
      P gene in hepatitis B virus infected patients resistant to lamivudine].
PG  - 616-8
AB  - OBJECTIVE: To explore the point mutation in hepatitis B virus polymerase (HBV P) 
      gene in HBV-infected patients resistant to lamivudine. METHODS: HBV P gene was 
      amplified by PCR and the products was sequenced to analyze the YMDD mutation. 
      Then the variants were detected by polymerase chain reaction-restriction fragment 
      length polymorphism (PCR-RFLP) with the following restriction enzymes: Fok I, Ssp 
      I, Alw441 and were separated by 8.0% polyacrylamide gel electrophoresis. RESULTS: 
      Comparing with the sequences of standard HBV genome, there were 16 patients with 
      G743C mutation and 1 patient with G743A mutation, and the codon ATG turned to ATC 
      and ATA, YMDD motif changed into YIDD. But this kind of YIDD mutation was not 
      proved by PCR-RFLP assay in the 17 patients. CONCLUSIONS: The G743C and G743A 
      mutations in HBV P gene, resulting in YMDD motif changed into YIDD, are detected 
      only by direct sequencing, not by PCR-RFLP. The new kind of G743C and G743A point 
      mutations in HBV P gene is important for the detection of HBV P gene YMDD 
      mutation.
FAU - Pan, Xiao-ping
AU  - Pan XP
AD  - Institute of Infectious Diseases, First Affiliated Hospital, Medical College of 
      Zhejiang University, Hangzhou 310003, China.
FAU - Wo, Jian-er
AU  - Wo JE
FAU - Chen, Zhi
AU  - Chen Z
FAU - Chen, Feng
AU  - Chen F
FAU - Liang, Wei-feng
AU  - Liang WF
FAU - Liu, Ke-zhou
AU  - Liu KZ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Gan Zang Bing Za Zhi
JT  - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of 
      hepatology
JID - 9710009
RN  - 0 (Antiviral Agents)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (Gene Products, pol)
RN  - 0 (P protein, Hepatitis B virus)
RN  - 2T8Q726O95 (Lamivudine)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
SB  - IM
MH  - Adult
MH  - Amino Acid Motifs/genetics
MH  - Antiviral Agents/pharmacology/therapeutic use
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - DNA, Viral/blood/genetics
MH  - DNA-Directed DNA Polymerase/genetics
MH  - Drug Resistance, Viral/*genetics
MH  - Female
MH  - Gene Products, pol/genetics
MH  - Hepatitis B virus/*genetics
MH  - Hepatitis B, Chronic/*drug therapy/virology
MH  - Humans
MH  - Lamivudine/pharmacology/*therapeutic use
MH  - Male
MH  - Middle Aged
MH  - *Point Mutation
EDAT- 2003/10/24 05:00
MHDA- 2005/03/16 09:00
CRDT- 2003/10/24 05:00
PHST- 2003/10/24 05:00 [pubmed]
PHST- 2005/03/16 09:00 [medline]
PHST- 2003/10/24 05:00 [entrez]
PST - ppublish
SO  - Zhonghua Gan Zang Bing Za Zhi. 2003 Oct;11(10):616-8.