PMID- 14572457 OWN - NLM STAT- MEDLINE DCOM- 20040123 LR - 20191108 IS - 1044-7431 (Print) IS - 1044-7431 (Linking) VI - 24 IP - 2 DP - 2003 Oct TI - Brain-derived neurotrophic factor upregulates and maintains AMPA receptor currents in neocortical GABAergic neurons. PG - 340-56 AB - The regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors is implicated in synaptic plasticity. Although we have found that brain-derived neurotrophic factor (BDNF) triggers surface translocation of AMPA receptor proteins, the physiological significance of the BDNF effect remained to be determined. The present immunohistochemical studies revealed that cortical GABAergic neurons exhibited the most striking response to BDNF. Accordingly, we monitored AMPA-triggered currents through GABAergic neurons: Chronic BDNF treatment increased the AMPA-triggered currents but not NMDA-triggered currents in culture. In parallel, the amplitude, but not frequency, of spontaneous miniature excitatory postsynaptic currents (mEPSCs) was elevated in GABAergic neurons. In agreement, BDNF enhanced GABA release triggered by AMPA compared to the amount triggered by high potassium. Conversely, there was a significant decrease in the mEPSC amplitude of GABAergic neurons in heterozygous BDNF-knockout mice. These findings indicate that the neurotrophin enhances the input sensitivity of GABAergic neurons to facilitate their inhibitory function in the neocortex. FAU - Nagano, T AU - Nagano T AD - Department of Molecular Neurobiology, Brain Research Institute, Niigata University, 951-8585 Nigata, Japan. FAU - Yanagawa, Y AU - Yanagawa Y FAU - Obata, K AU - Obata K FAU - Narisawa-Saito, M AU - Narisawa-Saito M FAU - Namba, H AU - Namba H FAU - Otsu, Y AU - Otsu Y FAU - Takei, N AU - Takei N FAU - Nawa, H AU - Nawa H LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Mol Cell Neurosci JT - Molecular and cellular neurosciences JID - 9100095 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Receptors, AMPA) RN - 56-12-2 (gamma-Aminobutyric Acid) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*deficiency/pharmacology MH - Cells, Cultured MH - Excitatory Postsynaptic Potentials/drug effects/physiology MH - Membrane Potentials/drug effects/physiology MH - Mice MH - Mice, Inbred C57BL MH - Mice, Knockout MH - Neocortex/drug effects/*metabolism MH - Neurons/drug effects/metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Receptors, AMPA/*biosynthesis MH - Up-Regulation/drug effects/*physiology MH - gamma-Aminobutyric Acid/*metabolism EDAT- 2003/10/24 05:00 MHDA- 2004/01/24 05:00 CRDT- 2003/10/24 05:00 PHST- 2003/10/24 05:00 [pubmed] PHST- 2004/01/24 05:00 [medline] PHST- 2003/10/24 05:00 [entrez] AID - S1044743103001726 [pii] AID - 10.1016/s1044-7431(03)00172-6 [doi] PST - ppublish SO - Mol Cell Neurosci. 2003 Oct;24(2):340-56. doi: 10.1016/s1044-7431(03)00172-6.