PMID- 14573813
OWN - NLM
STAT- MEDLINE
DCOM- 20031118
LR  - 20201208
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 84
IP  - Pt 11
DP  - 2003 Nov
TI  - Molecular characterization of a novel astrovirus associated with disease in mink.
PG  - 3087-3094
LID - 10.1099/vir.0.19267-0 [doi]
AB  - Pre-weaning diarrhoea is a well-known problem in mink farming in Europe, causing 
      morbidity that varies between farms, regions and season. Different causalities 
      for the disease have been proposed, but only most recently has a novel astrovirus 
      been identified as an important risk factor. In this report, the molecular 
      characterization, origin and evolution of this novel astrovirus of mink are 
      discussed. The polyadenylated, positive-stranded RNA genome was sequenced and 
      found to contain 6610 nt, organized into three ORFs and two short UTRs. A 
      ribosomal frameshift sequence links the 5' two ORFs, containing sequence motifs 
      for a serine protease (ORF1a) and an RNA-dependent RNA polymerase (ORF1b). The 
      structural proteins are encoded by ORF2 and, presumably, are expressed as a 
      polyprotein precursor to be cleaved into the mature capsid proteins. These 
      results indicate that mink astrovirus (MiAstV) has all of the features typical of 
      members of the Astroviridae. Phylogenetic analyses revealed that MiAstV is 
      distantly related to established astroviruses, showing less than 67 % similarity 
      at the nucleotide level with its closest relative, ovine astrovirus, and even 
      lower identities at the predicted amino acid level. Nevertheless, sequence 
      analysis of MiAstV isolates from geographically distinct Swedish and Danish farms 
      showed much less diversity. This suggests either the spread in the mink 
      population of a virus that has evolved a long time ago or the recent introduction 
      of an ancient virus into a new host species.
FAU - Mittelholzer, Christian
AU  - Mittelholzer C
AD  - Department of Virology, Swedish Institute for Infectious Disease Control, 
      S-171 82 Solna, Sweden.
FAU - Hedlund, Kjell-Olof
AU  - Hedlund KO
AD  - Department of Molecular Epidemiology and Biotechnology, Swedish Institute for 
      Infectious Disease Control, S-171 82 Solna, Sweden.
FAU - Englund, Lena
AU  - Englund L
AD  - Department of Small Animals, National Veterinary Institute, S-751 89 Uppsala, 
      Sweden.
FAU - Dietz, Hans-Henrik
AU  - Dietz HH
AD  - Department of Poultry, Fish and Fur Animals, Danish Veterinary Institute, DK-8200 
      Arhus, Denmark.
FAU - Svensson, Lennart
AU  - Svensson L
AD  - Department of Virology, Swedish Institute for Infectious Disease Control, 
      S-171 82 Solna, Sweden.
LA  - eng
SI  - GENBANK/AY179509
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (RNA, Viral)
RN  - EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Mamastrovirus/classification/*genetics
MH  - Mink/*virology
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Phylogeny
MH  - RNA, Viral/analysis
MH  - RNA-Dependent RNA Polymerase/genetics
MH  - Serine Endopeptidases/genetics
EDAT- 2003/10/24 05:00
MHDA- 2003/12/03 05:00
CRDT- 2003/10/24 05:00
PHST- 2003/10/24 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/10/24 05:00 [entrez]
AID - 10.1099/vir.0.19267-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2003 Nov;84(Pt 11):3087-3094. doi: 10.1099/vir.0.19267-0.