PMID- 14576049
OWN - NLM
STAT- MEDLINE
DCOM- 20040511
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 103
IP  - 7
DP  - 2004 Apr 1
TI  - DC-SIGN promotes exogenous MHC-I-restricted HIV-1 antigen presentation.
PG  - 2648-54
AB  - Dendritic cells (DCs) facilitate HIV-1 spread in the host by capturing virions 
      and transferring them to permissive lymphocytes in lymphoid organs. Lectins such 
      as DC-specific ICAM-grabbing non-integrin (DC-SIGN) are involved in HIV-1 uptake 
      by DCs, through high-affinity binding to viral envelope glycoproteins. We 
      examined the role of DC-SIGN on the fate of incoming virions and on major 
      histocompatibility complex class I (MHC-I)-restricted HIV-1 antigen presentation. 
      We show that DC-SIGN expression in B-cell lines dramatically enhances viral 
      internalization. In these cells, and also in primary DCs, most of the captured 
      virions are rapidly degraded, likely in a lysosomal compartment. In addition, a 
      fraction of incoming viral material is processed by the proteasome, leading to 
      activation of anti-HIV-specific cytotoxic T lymphocytes (CTLs) by 
      DC-SIGN-expressing cells. In DCs, DC-SIGN is not the only receptor involved, and 
      redundant pathways of virus capture leading to antigen presentation likely 
      coexist. Altogether, our results highlight new aspects of DC-SIGN interactions 
      with HIV-1. The lectin does not significantly protect captured virions against 
      degradation and promotes MHC-I exogenous presentation of HIV-1 antigens.
FAU - Moris, Arnaud
AU  - Moris A
AD  - Groupe Virus et Immunite, URA CNRS 1930, Institut Pasteur, Paris, France.
FAU - Nobile, Cinzia
AU  - Nobile C
FAU - Buseyne, Florence
AU  - Buseyne F
FAU - Porrot, Francoise
AU  - Porrot F
FAU - Abastado, Jean-Pierre
AU  - Abastado JP
FAU - Schwartz, Olivier
AU  - Schwartz O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031023
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (CD4 Antigens)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (HIV Antigens)
RN  - 0 (HIV Core Protein p24)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Antigen Presentation/*immunology
MH  - Antigens, CD/immunology
MH  - CD4 Antigens/immunology
MH  - Cell Adhesion Molecules/*immunology
MH  - Dendritic Cells/cytology/*immunology
MH  - HIV Antigens/*immunology
MH  - HIV Core Protein p24/immunology
MH  - HIV-1/*immunology
MH  - HLA-A2 Antigen/immunology
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Humans
MH  - Lectins, C-Type/*immunology
MH  - Major Histocompatibility Complex
MH  - Receptors, Cell Surface/*immunology
MH  - Virion/immunology
EDAT- 2003/10/25 05:00
MHDA- 2004/05/12 05:00
CRDT- 2003/10/25 05:00
PHST- 2003/10/25 05:00 [pubmed]
PHST- 2004/05/12 05:00 [medline]
PHST- 2003/10/25 05:00 [entrez]
AID - S0006-4971(20)43874-1 [pii]
AID - 10.1182/blood-2003-07-2532 [doi]
PST - ppublish
SO  - Blood. 2004 Apr 1;103(7):2648-54. doi: 10.1182/blood-2003-07-2532. Epub 2003 Oct 
      23.