PMID- 14578359
OWN - NLM
STAT- MEDLINE
DCOM- 20040303
LR  - 20211203
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 279
IP  - 1
DP  - 2004 Jan 2
TI  - FKBP12-rapamycin-associated protein or mammalian target of rapamycin (FRAP/mTOR) 
      localization in the endoplasmic reticulum and the Golgi apparatus.
PG  - 772-8
AB  - FKBP12-rapamycin-associated protein (FRAP) or mammalian target of rapamycin 
      (mTOR) and its effector proteins form a critical signaling pathway that regulates 
      eukaryotic cell growth and proliferation. Although the protein components in this 
      pathway have begun to be identified, little is known about their subcellular 
      localization or the physiological significance of their localization. By 
      immunofluorescence, we find that both endogenous and recombinant FRAP/mTOR 
      proteins show localization predominantly in the endoplasmic reticulum (ER) and 
      the Golgi apparatus. Consistent with this finding, FRAP/mTOR is cofractionated 
      with calnexin, an ER marker protein. Biochemical characterization suggests that 
      FRAP/mTOR is a peripheral ER/Golgi protein with tight membrane association. 
      Finally, we have identified domains of FRAP/mTOR which may mediate its 
      association with the ER and the Golgi apparatus.
FAU - Drenan, Ryan M
AU  - Drenan RM
AD  - Molecular Cell Biology Graduate Program, Washington University School of 
      Medicine, St. Louis, Missouri 63110, USA.
FAU - Liu, Xiangyu
AU  - Liu X
FAU - Bertram, Paula G
AU  - Bertram PG
FAU - Zheng, X F Steven
AU  - Zheng XF
LA  - eng
GR  - R01CA099004/CA/NCI NIH HHS/United States
GR  - R01CA77668/CA/NCI NIH HHS/United States
GR  - R01GM62817/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20031024
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 5.2.1.- (Tacrolimus Binding Protein 1A)
SB  - IM
MH  - Animals
MH  - *Carrier Proteins
MH  - Endoplasmic Reticulum/*physiology
MH  - Golgi Apparatus/*physiology
MH  - HeLa Cells
MH  - Humans
MH  - Mammals
MH  - Phosphotransferases (Alcohol Group Acceptor)/analysis/*physiology
MH  - Protein Kinases/analysis/*physiology
MH  - Recombinant Proteins/analysis/metabolism
MH  - TOR Serine-Threonine Kinases
MH  - Tacrolimus Binding Protein 1A/analysis/*physiology
MH  - Transfection
EDAT- 2003/10/28 05:00
MHDA- 2004/03/05 05:00
CRDT- 2003/10/28 05:00
PHST- 2003/10/28 05:00 [pubmed]
PHST- 2004/03/05 05:00 [medline]
PHST- 2003/10/28 05:00 [entrez]
AID - S0021-9258(18)52872-6 [pii]
AID - 10.1074/jbc.M305912200 [doi]
PST - ppublish
SO  - J Biol Chem. 2004 Jan 2;279(1):772-8. doi: 10.1074/jbc.M305912200. Epub 2003 Oct 
      24.