PMID- 14579127
OWN - NLM
STAT- MEDLINE
DCOM- 20040728
LR  - 20181113
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 112
IP  - 3
DP  - 2003 Oct
TI  - Rearrangements of ribosomal DNA clusters in late generation telomerase-deficient 
      Arabidopsis.
PG  - 116-23
AB  - The ends of eukaryotic chromosomes are capped with special nucleoprotein 
      structures called telomeres. Telomere shortening due to telomerase inactivation 
      may result in fusion of homologous or heterologous chromosomes, leading to their 
      successive breakage during anaphase movement, followed by fusion of broken ends 
      in the next cell cycle, i.e. the breakage-fusion-bridge (BFB) cycle. Using 
      fluorescence in situ hybridization (FISH) with 25S rDNA and specific bacterial 
      artificial chromosome (BAC) probes we demonstrate participation of chromosomes 2 
      and 4 of Arabidopsis thaliana AtTERT null plants in the formation of anaphase 
      bridges. Both homologous and non-homologous chromosomes formed transient anaphase 
      bridges whose breakage and unequal separation led to genome rearrangement, 
      including non-reciprocal translocations and aneuploidy. The 45S rDNA regions 
      located at the ends of chromosomes 2 and 4 were observed in chromosome bridges at 
      a frequency approximately ten times higher than expected in the case of random 
      fusion events. This outcome could result from a functional association of rDNA 
      repeats at nucleoli. We also describe increased variation in the number of 
      nucleoli in some interphase cells with supernumerary rDNA FISH signals. These 
      data indicate that dysfunctional telomeres in Arabidopsis lead to massive genome 
      instability, which is induced by multiple rounds of the BFB mechanism.
FAU - Siroky, Jiri
AU  - Siroky J
AD  - Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy of 
      Sciences of the Czech Republic, Kralovopolska 135, 612 65 Brno, Czech Republic.
FAU - Zluvova, Jitka
AU  - Zluvova J
FAU - Riha, Karel
AU  - Riha K
FAU - Shippen, Dorothy E
AU  - Shippen DE
FAU - Vyskot, Boris
AU  - Vyskot B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20030917
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (DNA, Ribosomal)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Anaphase/genetics
MH  - Arabidopsis/*genetics
MH  - Cell Nucleolus/metabolism
MH  - Chromosomes, Artificial, Bacterial
MH  - Chromosomes, Plant/genetics/*metabolism
MH  - DNA, Ribosomal/genetics
MH  - Gene Rearrangement/*genetics
MH  - *Genomic Instability
MH  - In Situ Hybridization, Fluorescence
MH  - Telomerase/*deficiency
MH  - Telomere/genetics/*metabolism
EDAT- 2003/10/28 05:00
MHDA- 2004/07/29 05:00
CRDT- 2003/10/28 05:00
PHST- 2003/04/18 00:00 [received]
PHST- 2003/07/07 00:00 [accepted]
PHST- 2003/10/28 05:00 [pubmed]
PHST- 2004/07/29 05:00 [medline]
PHST- 2003/10/28 05:00 [entrez]
AID - 10.1007/s00412-003-0251-7 [doi]
PST - ppublish
SO  - Chromosoma. 2003 Oct;112(3):116-23. doi: 10.1007/s00412-003-0251-7. Epub 2003 Sep 
      17.