PMID- 14579539
OWN - NLM
STAT- MEDLINE
DCOM- 20031211
LR  - 20061115
IS  - 0379-4172 (Print)
IS  - 0379-4172 (Linking)
VI  - 30
IP  - 7
DP  - 2003 Jul
TI  - [Random amplified polymorphic DNA (RAPD) analysis of Drosophila simulans in the 
      mainland of China].
PG  - 673-80
AB  - The widespread distribution of Drosophila simulans in the mainland of China was 
      found recently. Random amplified polymorphic DNA (RAPD) was used to analyzed DNA 
      polymorphisms of 38 Drosophila simulans populations collected from the mainland 
      of China. The origins of D. simulans in China were also discussed. Using 40 
      arbitary primers (10 bp), we made PCR amplifications under the optimized reaction 
      conditions for RAPD that had been established in our laboratory. Our molecular 
      phylogenetic tree constructed by UPGMA method showed as follows: (1) 38 
      populations are apparently classified into Northern cluster and Southern cluster, 
      where NJ(Nanjing) can be considered as the boundary according to latitude. The 
      populations in the Northern cluster can be further grouped into two sub-clusters, 
      where BJ(Beijing) can be considered as the boundary according to latitude. The 
      Northeast one consists of 10 populations, including Mohe(MH), Hailaer(HLR), 
      Heihe(HH), Jiamusi(JMS), Haerbin(HRB), Changchun(CC), Shenyang(SY), Dandong(DD), 
      Yanji(YJ), Tumen(TM); the other consists of 12 populations, including Beijing 
      (BJ), Datong(DT), Huhehaote(HHHT), Yinchun(YC), Xinin(XN), Lanzhou(LZ), 
      Taiyuan(TY), Shijiazhuang(SJZ), Yantai (YT), Jinan(JN), Xuzhou(XZ), 
      Lianyungang(LYG). The southern cluster consists of the left 16 populations, 
      including Zhengzhou (ZZ), Wuhan(WH), Shanghai(SH), Nanjing(NJ), Hangzhou(HZ), 
      Chongqing(CQ), Nanchang(NC), Wenzhou(WZ), Changsha (CS), Guiyang(GY), Fuzhou(FZ), 
      Kunming(KM), Xiamen(XM), Guangzhou(GZ), Nanning(NN), Hainan(HN). (2) The genetic 
      distances among populations relate to the collection sites to a great extent, 
      though there are some exceptions. According to the facts that we know, (1) D. 
      simulans is an exotic species; (2) The invasion of D. simulans in the mainland of 
      China took place in recent 30 years or so. (3) The widespread distribution of D. 
      simulans in the mainland of China started in recent 10 years or so. Therefore, 
      the results above might not be due to geographical differentiation, but due to 
      genetic differences of initial founder individual(s) between geographical 
      populations. Some of geographically adjacent populations, however, have common 
      initial founder individual(s), which results in the classification of D. simulans 
      populations according to their geographical relationship. We suggest that there 
      are two possible reasons which result in the genetic differences of initial 
      founder individual(s) between geographical populations, one is the D. simulans in 
      the mainland of China had multiple origins, the other is founder effect and/or 
      bottleneck effect that the D. simulans have experienced in the course of 
      expansion.
FAU - Xu, Shu-Hua
AU  - Xu SH
AD  - Faculty of Life Science, Hubei University, Wuhan 430062, China.
FAU - Zeng, Qing-Tao
AU  - Zeng QT
FAU - Qian, Yuan-Huai
AU  - Qian YH
FAU - Li, Shou-Tao
AU  - Li ST
FAU - Yang, Yong
AU  - Yang Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan Xue Bao
JT  - Yi chuan xue bao = Acta genetica Sinica
JID - 7900784
SB  - IM
MH  - Animals
MH  - China
MH  - Drosophila/classification/*genetics
MH  - Phylogeny
MH  - *Random Amplified Polymorphic DNA Technique
EDAT- 2003/10/29 05:00
MHDA- 2003/12/12 05:00
CRDT- 2003/10/29 05:00
PHST- 2003/10/29 05:00 [pubmed]
PHST- 2003/12/12 05:00 [medline]
PHST- 2003/10/29 05:00 [entrez]
PST - ppublish
SO  - Yi Chuan Xue Bao. 2003 Jul;30(7):673-80.