PMID- 14580651
OWN - NLM
STAT- MEDLINE
DCOM- 20040316
LR  - 20190823
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 60
IP  - 9
DP  - 2003 Dec
TI  - The effect of follicle-stimulating hormone on follicular development, granulosa 
      cell apoptosis and steroidogenesis and its mediation by insulin-like growth 
      factor-I in the goat ovary.
PG  - 1691-704
AB  - The effect of FSH on goat follicular development, granulosa cell apoptosis and 
      steroidogenesis and its mediation by insulin-like growth factor (IGF)-I were 
      studied through both in vivo and in vitro experiments. The FSH treatment was 
      begun on Day 9 after estrus and consisted of injections twice a day for 3 days in 
      decreasing doses (7.5-7.5-5.0-5.0-2.5-2.5 mg). Does in both treatment and control 
      groups were slaughtered for ovaries on Day 12. Granulosa cell apoptosis was 
      detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling 
      (TUNEL). Expression of IGF-I and IGF-II mRNA was determined by RT-PCR, while 
      concentrations of progesterone (P4), estradiol (E2), IGF-I and IGF-II were 
      measured by radioimmunoassay (RIA). Following parameters increased significantly 
      (P<0.05) after the FSH treatment: follicle number (5.0+/-1.5 versus 9.0+/-2.0 per 
      ovary), the level of E2 (0.1+/-0.1 ng/ml versus 0.7+/-0.2 ng/ml), the E2/P4 ratio 
      (0.7+/-0.4 versus 4.7+/-3.0) and the concentrations of IGF-I (0.5+/-0.2 ng/ml 
      versus 119.4+/-15.1 ng/ml) and IGF-II (0.12+/-0.03 ng/ml versus 40.9+/-18.7 
      ng/ml) in follicular fluid of the medium sized (3-5 mm) follicles and in the 
      ovarian cortex the relative quantity of IGF-I mRNA (0.37+/-0.17 versus 
      0.90+/-0.12 Max OD). In contrast, the ratio of apoptotic granulosa cells in these 
      follicles was reduced significantly (0.53+/-0.1 versus 0.10+/-0.01, P<0.05). In 
      large (>5 mm) follicles, however, only the follicle number (2.3+/-0.7 versus 
      7.0+/-1.5 per ovary) and the level of IGF-I (38.4+/-11.0 ng/ml versus 87.3+/-13.9 
      ng/ml) increased significantly (P<0.05), whereas other values did not change. In 
      vitro culture of granulosa cells showed that FSH significantly (P<0.05) enhanced 
      IGF-I production (12.7+/-2.1 ng/ml versus 26.+/-21.9 ng/ml) by these cells, and 
      both FSH and IGF-I reduced the ratios of apoptotic cells (from 0.7+/-0.07 to 
      0.3+/-0.1 and 0.2+/-0.04, respectively) and the effect was additive when both 
      were used together. H89, the PKA pathway inhibitor, blocked the effect of FSH on 
      granulosa cell apoptosis and IGF-I production in vitro. These results indicated 
      that FSH mainly enhanced the development of medium sized follicles in the goat by 
      suppressing the apoptosis of granulosa cells via increasing production of IGF-I 
      and steroids, possibly through the PKA pathway.
FAU - Yu, Yuansong
AU  - Yu Y
AD  - College of Animal Science and Veterinary Medicine, Shandong Agricultural 
      University, Tai-an City 271018, PR China.
FAU - Li, Wei
AU  - Li W
FAU - Han, Zhengbin
AU  - Han Z
FAU - Luo, Mingjiu
AU  - Luo M
FAU - Chang, Zhongle
AU  - Chang Z
FAU - Tan, Jinghe
AU  - Tan J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Steroids)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 67763-97-7 (Insulin-Like Growth Factor II)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects/physiology
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Follicle Stimulating Hormone/*pharmacology
MH  - Goats/metabolism/*physiology
MH  - Granulosa Cells/drug effects/pathology
MH  - In Situ Nick-End Labeling/veterinary
MH  - Insulin-Like Growth Factor I/metabolism
MH  - Insulin-Like Growth Factor II/metabolism
MH  - Ovarian Follicle/drug effects/*physiology
MH  - Ovary/physiology
MH  - Radioimmunoassay/veterinary
MH  - Random Allocation
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Steroids/*biosynthesis
EDAT- 2003/10/29 05:00
MHDA- 2004/03/18 05:00
CRDT- 2003/10/29 05:00
PHST- 2003/10/29 05:00 [pubmed]
PHST- 2004/03/18 05:00 [medline]
PHST- 2003/10/29 05:00 [entrez]
AID - S0093691X03002887 [pii]
AID - 10.1016/j.theriogenology.2003.08.001 [doi]
PST - ppublish
SO  - Theriogenology. 2003 Dec;60(9):1691-704. doi: 
      10.1016/j.theriogenology.2003.08.001.