PMID- 14580783
OWN - NLM
STAT- MEDLINE
DCOM- 20031224
LR  - 20190819
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 192
IP  - 2-3
DP  - 2003 Nov 5
TI  - Impact of dietary exposure to methoxychlor, genistein, or diisononyl phthalate 
      during the perinatal period on the development of the rat endocrine/reproductive 
      systems in later life.
PG  - 149-70
AB  - To evaluate the impact of dietary exposure to endocrine disrupting chemicals 
      (EDCs) during the sensitive period of brain sexual differentiation, maternal 
      Sprague-Dawley rats were fed three representative chemicals, methoxychlor (MXC; 
      24, 240, and 1200 ppm), genistein (GEN; 20, 200, and 1000 ppm), or diisononyl 
      phthalate (DINP; 400, 4000, and 20,000 ppm), from gestational day 15 to postnatal 
      day 10. Soy-free diet was used as a basal diet to eliminate possible estrogenic 
      effects from the standard diet. Offspring were examined in terms of anogenital 
      distances, prepubertal organ weights, onset of puberty, estrous cyclicity, and 
      organ weights and histopathology of endocrine organs at adult stage (week 11) as 
      well as the volumes of sexually dimorphic nucleus of preoptic area (SDN-POA). All 
      chemicals caused signs of maternal toxicity at high doses. MXC, at 1200 ppm, 
      facilitated and delayed the onset of puberty in females and males, respectively, 
      females also showing endocrine disrupting effects thereafter, such as irregular 
      estrous cyclicity and histopathological alterations in the reproductive tract and 
      anterior pituitary. GEN, at all doses, reduced body weight (BW) at week 11, but 
      did not affect endocrine parameters. Treatment with DINP at 20,000 ppm resulted 
      in degeneration of meiotic spermatocytes and Sertoli cells in the testis and 
      decrease of corpora lutea in the ovary at week 11, although changes remained 
      minimal or slight. The SDN-POA volume remained unchanged with all three 
      chemicals. The results demonstrated that perinatal dietary exposure to EDCs for a 
      limited period causes endocrine disruption in offspring only at high doses.
FAU - Masutomi, Naoya
AU  - Masutomi N
AD  - Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 
      Setagaya-ku, Tokyo 158-8501, Japan.
FAU - Shibutani, Makoto
AU  - Shibutani M
FAU - Takagi, Hironori
AU  - Takagi H
FAU - Uneyama, Chikako
AU  - Uneyama C
FAU - Takahashi, Noriyuki
AU  - Takahashi N
FAU - Hirose, Masao
AU  - Hirose M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Phthalic Acids)
RN  - 4010KIX4CK (diisononyl phthalate)
RN  - DH2M523P0H (Genistein)
RN  - RIA79UD69L (Methoxychlor)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Body Weight/drug effects
MH  - Endocrine System/*drug effects/growth & development
MH  - Estrous Cycle/drug effects
MH  - Female
MH  - Genistein/*toxicity
MH  - Male
MH  - Methoxychlor/*toxicity
MH  - Organ Size/drug effects
MH  - Ovary/drug effects/growth & development/pathology
MH  - Phthalic Acids/*toxicity
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Preoptic Area/drug effects/growth & development/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reproduction/*drug effects
MH  - Sex Differentiation/drug effects
MH  - Testis/drug effects/growth & development/pathology
EDAT- 2003/10/29 05:00
MHDA- 2003/12/25 05:00
CRDT- 2003/10/29 05:00
PHST- 2003/10/29 05:00 [pubmed]
PHST- 2003/12/25 05:00 [medline]
PHST- 2003/10/29 05:00 [entrez]
AID - S0300483X03002695 [pii]
AID - 10.1016/s0300-483x(03)00269-5 [doi]
PST - ppublish
SO  - Toxicology. 2003 Nov 5;192(2-3):149-70. doi: 10.1016/s0300-483x(03)00269-5.