PMID- 14581123
OWN - NLM
STAT- MEDLINE
DCOM- 20040113
LR  - 20220408
IS  - 0304-3959 (Print)
IS  - 1872-6623 (Electronic)
IS  - 0304-3959 (Linking)
VI  - 106
IP  - 1-2
DP  - 2003 Nov
TI  - Joint manipulation reduces hyperalgesia by activation of monoamine receptors but 
      not opioid or GABA receptors in the spinal cord.
PG  - 159-68
AB  - Joint manipulation has long been used for pain relief. However, the underlying 
      mechanisms for manipulation-related pain relief remain largely unexplored. The 
      purpose of the current study was to determine which spinal neurotransmitter 
      receptors mediate manipulation-induced antihyperalgesia. Rats were injected with 
      capsaicin (50 microl, 0.2%) into one ankle joint and mechanical withdrawal 
      threshold measured before and after injection. The mechanical withdrawal 
      threshold decreases 2 h after capsaicin injection. Two hours after capsaicin 
      injection, the following drugs were administered intrathecally: bicuculline, 
      blocks gamma-aminobutyric acid (GABAA) receptors; naloxone, blocks opioid 
      receptors; yohimbine blocks, alpha2-adrenergic receptors; and methysergide, 
      blocks 5-HT(1/2) receptors. In addition, NAN-190, ketanserin, and MDL-72222 were 
      administered to selectively block 5-HT1A, 5-HT2A, and 5-HT3 receptors, 
      respectively. Knee joint manipulation was performed 15 min after administration 
      of drug. The knee joint was flexed and extended to end range of extension while 
      the tibia was simultaneously translated in an anterior to posterior direction. 
      The treatment group received three applications of manipulation, each 3 min in 
      duration separated by 1 min of rest. Knee joint manipulation after capsaicin 
      injection into the ankle joint significantly increases the mechanical withdrawal 
      threshold for 45 min after treatment. Spinal blockade of 5-HT(1/2) receptors with 
      methysergide prevented, while blockade of alpha2-adrenergic receptors attenuated, 
      the manipulation-induced antihyperalgesia. NAN-190 also blocked 
      manipulation-induced antihyperalgesia suggesting that effects of methysergide are 
      mediated by 5-HT1A receptor blockade. However, spinal blockade of opioid or GABAA 
      receptors had no effect on manipulation induced-antihyperalgesia. Thus, the 
      antihyperalgesia produced by joint manipulation appears to involve descending 
      inhibitory mechanisms that utilize serotonin and noradrenaline.
FAU - Skyba, D A
AU  - Skyba DA
AD  - Neuroscience Graduate Program, University of Iowa, Iowa City, IA, USA.
FAU - Radhakrishnan, R
AU  - Radhakrishnan R
FAU - Rohlwing, J J
AU  - Rohlwing JJ
FAU - Wright, A
AU  - Wright A
FAU - Sluka, K A
AU  - Sluka KA
LA  - eng
GR  - K02 AR002201-05/AR/NIAMS NIH HHS/United States
GR  - KO2 AR 02201/AR/NIAMS NIH HHS/United States
GR  - K02 AR002201-01A1/AR/NIAMS NIH HHS/United States
GR  - R21 AT 001130-02/AT/NCCIH NIH HHS/United States
GR  - K02 AR002201-03/AR/NIAMS NIH HHS/United States
GR  - K02 AR002201/AR/NIAMS NIH HHS/United States
GR  - R21 AT001130-01/AT/NCCIH NIH HHS/United States
GR  - K02 AR002201-02/AR/NIAMS NIH HHS/United States
GR  - K02 AR002201-04/AR/NIAMS NIH HHS/United States
GR  - R21 AT001130-02/AT/NCCIH NIH HHS/United States
GR  - R21 AT001130/AT/NCCIH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Pain
JT  - Pain
JID - 7508686
RN  - 0 (Adrenergic alpha-Antagonists)
RN  - 0 (GABA Antagonists)
RN  - 0 (Narcotic Antagonists)
RN  - 0 (Receptors, Adrenergic)
RN  - 0 (Receptors, GABA)
RN  - 0 (Receptors, Opioid)
RN  - 0 (Receptors, Serotonin)
RN  - 0 (Serotonin Antagonists)
RN  - 2Y49VWD90Q (Yohimbine)
RN  - 36B82AMQ7N (Naloxone)
RN  - XZA9HY6Z98 (Methysergide)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
MH  - Adrenergic alpha-Antagonists/pharmacology
MH  - Animals
MH  - Bicuculline/pharmacology
MH  - GABA Antagonists/pharmacology
MH  - Hyperalgesia/*physiopathology/*therapy
MH  - Joints/physiology
MH  - Male
MH  - Methysergide/pharmacology
MH  - Naloxone/pharmacology
MH  - Narcotic Antagonists/pharmacology
MH  - Pain/physiopathology
MH  - Pain Management
MH  - Physical Stimulation
MH  - *Physical Therapy Modalities
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Adrenergic/*physiology
MH  - Receptors, GABA/physiology
MH  - Receptors, Opioid/physiology
MH  - Receptors, Serotonin/*physiology
MH  - Serotonin Antagonists/pharmacology
MH  - Spinal Cord/*physiology
MH  - Yohimbine/pharmacology
PMC - PMC2732015
MID - NIHMS66956
EDAT- 2003/10/29 05:00
MHDA- 2004/01/14 05:00
PMCR- 2009/08/26
CRDT- 2003/10/29 05:00
PHST- 2003/10/29 05:00 [pubmed]
PHST- 2004/01/14 05:00 [medline]
PHST- 2003/10/29 05:00 [entrez]
PHST- 2009/08/26 00:00 [pmc-release]
AID - S0304395903003208 [pii]
AID - 10.1016/s0304-3959(03)00320-8 [doi]
PST - ppublish
SO  - Pain. 2003 Nov;106(1-2):159-68. doi: 10.1016/s0304-3959(03)00320-8.