PMID- 14581147
OWN - NLM
STAT- MEDLINE
DCOM- 20040712
LR  - 20221207
IS  - 0168-8227 (Print)
IS  - 0168-8227 (Linking)
VI  - 62
IP  - 2
DP  - 2003 Nov
TI  - The Pro12Ala polymorphism of PPARgamma2 gene and susceptibility to type 2 
      diabetes mellitus in a Polish population.
PG  - 105-11
AB  - INTRODUCTION: It has recently been shown that polymorphisms of some genes might 
      influence the genetic susceptibility to complex, multifactorial forms of type 2 
      diabetes mellitus (T2DM). One of those genes is peroxisome proliferator activated 
      receptor gamma (PPARgamma). The PPARgamma gene product is a nuclear hormone 
      receptor that regulates adipogenesis and is a target for thiazolidinediones, 
      medications enhancing sensitivity to insulin. The Pro12Ala amino acid variant of 
      the PPARgamma2 isoform is associated with T2DM in several populations. AIMS: (1) 
      To determine the allele and genotype frequency of the Pro12Ala PPARgamma2 amino 
      acid variant in a Polish population; (2) To search for the association of the 
      Pro12Ala polymorphism with T2DM in the examined population. METHODS: We included 
      644 individuals in this study: 366 T2DM patients with age of diagnosis greater 
      than 35 years and 278 non-diabetic control subjects. The fragment of the 
      PPARgamma2 gene which contains the examined amino acid variant was amplified by 
      polymerase chain reaction (PCR). Alleles and genotypes were determined based on 
      electrophoresis of the DNA digestion products by the specific restriction enzyme 
      BshI. Differences in distribution between the groups were examined by chi2 test. 
      RESULTS: The frequency of Pro/Ala alleles was similar in T2DM patients and in the 
      control subjects (83.5%/16.5% vs. 84.5%/15.5%, respectively, P=0.607). Similarly, 
      there was no difference between the groups when we analysed the genotype 
      distribution. Stratification analyses based on age of diagnosis, body mass index 
      (BMI), and family history of T2DM were performed. The Pro/Ala and Ala/Ala 
      genotypes tended to be more frequent in T2DM cases with age of diagnosis >50 
      years than in controls (36.2% vs. 27.3%, P=0.046). This difference was not 
      significant after Sheffe correction for multiple comparisons. The other 
      stratification analyses did not show any difference between the groups. 
      CONCLUSION: The frequency of the Pro12Ala PPARgamma2 polymorphism in the Polish 
      population studied is similar to that in other Caucasian populations. In the 
      case-control study, we were not able to confirm earlier reports that the Pro 
      allele conferred an increased risk for development of T2DM. Moreover, the results 
      of the stratified analysis suggest an opposite trend in late onset T2DM.
FAU - Malecki, Maciej T
AU  - Malecki MT
AD  - Department of Metabolic Diseases, Medical College, Jagiellonian University, 15 
      Kopernika Street, 31-501 Krakow, Poland. mmalecki@cm-uj.krakow.pl
FAU - Frey, Jakub
AU  - Frey J
FAU - Klupa, Tomasz
AU  - Klupa T
FAU - Skupien, Jan
AU  - Skupien J
FAU - Walus, Malgorzata
AU  - Walus M
FAU - Mlynarski, Wojciech
AU  - Mlynarski W
FAU - Sieradzki, Jacek
AU  - Sieradzki J
LA  - eng
GR  - CA 1 R03 TW01351-01/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Diabetes Res Clin Pract
JT  - Diabetes research and clinical practice
JID - 8508335
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Transcription Factors)
RN  - 9DLQ4CIU6V (Proline)
RN  - OF5P57N2ZX (Alanine)
SB  - IM
MH  - Age of Onset
MH  - Alanine
MH  - Amino Acid Substitution
MH  - Body Mass Index
MH  - Diabetes Mellitus, Type 2/*genetics
MH  - Genetic Predisposition to Disease/genetics
MH  - Genotype
MH  - Glycated Hemoglobin/analysis
MH  - Humans
MH  - Middle Aged
MH  - Poland
MH  - *Polymorphism, Restriction Fragment Length
MH  - Proline
MH  - Protein Isoforms/genetics
MH  - Receptors, Cytoplasmic and Nuclear/*genetics
MH  - Reference Values
MH  - Transcription Factors/*genetics
MH  - White People
EDAT- 2003/10/29 05:00
MHDA- 2004/07/13 05:00
CRDT- 2003/10/29 05:00
PHST- 2003/10/29 05:00 [pubmed]
PHST- 2004/07/13 05:00 [medline]
PHST- 2003/10/29 05:00 [entrez]
AID - S0168822703001645 [pii]
AID - 10.1016/s0168-8227(03)00164-5 [doi]
PST - ppublish
SO  - Diabetes Res Clin Pract. 2003 Nov;62(2):105-11. doi: 
      10.1016/s0168-8227(03)00164-5.