PMID- 14582133
OWN - NLM
STAT- MEDLINE
DCOM- 20040206
LR  - 20220310
IS  - 1552-4949 (Print)
IS  - 1552-4949 (Linking)
VI  - 56
IP  - 1
DP  - 2003 Nov
TI  - Comparative measurement of spontaneous apoptosis in pediatric acute leukemia by 
      different techniques.
PG  - 16-22
AB  - BACKGROUND: To distinguish between subgroups of patients with acute leukemia, the 
      rate of spontaneous (culture-induced) apoptosis of leukemic cells was evaluated 
      using five methods. METHODS: Leukemic cells (cells) from the bone marrow of 
      children with acute lymphoblastic leukemia (ALL, n = 112) and acute myeloid 
      leukemia (AML, n = 30) were cultured for 20 h in vitro. The level of apoptosis 
      was detected by fluorescent microscopy after staining with acridine orange (AO) 
      or by flow cytometry after staining using PI, JC-1, the APO-BRDU kit, or the 
      AnnexinV-FITC kit. RESULTS: ALL cells were significantly more sensitive to 
      spontaneous apoptosis versus AML cells, as was detected by all methods. The least 
      sensitive technique was apoptosis detection by sub-G1-peak/PI-staining. No 
      difference in the rate of apoptosis in cells was determined between T- and 
      B-lineage ALL patients. In patients with B-lineage ALL, strong positive 
      correlation existed between the level of cells with loss of mitochondrial 
      membrane potential (JC-1), chromatin condensation (AO), and externalization of 
      phosphatidylserine (AnnexinV+PI+). The proportion of AnnexinV+PI- cells had no 
      correlative link with any other apoptotic cell subpopulation. CONCLUSIONS: We 
      found different sensitivities of ALL and AML cells to undergoing spontaneous 
      apoptosis in vitro. Detection of the early/intermediate, but not the late stage 
      of apoptosis is of preferable for correct assignment of spontaneous apoptosis in 
      pediatric acute leukemia.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Savitskiy, Valery P
AU  - Savitskiy VP
AD  - Belarussian Center for Pediatric Oncology & Hematology, Minsk, Belarus.
FAU - Shman, Tatiana V
AU  - Shman TV
FAU - Potapnev, Michael P
AU  - Potapnev MP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry B Clin Cytom
JT  - Cytometry. Part B, Clinical cytometry
JID - 101235690
SB  - IM
MH  - Adolescent
MH  - Apoptosis/*physiology
MH  - B-Lymphocytes/cytology
MH  - Cell Lineage/immunology
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunophenotyping/*methods
MH  - In Vitro Techniques
MH  - Infant
MH  - Leukemia, Myeloid, Acute/*pathology
MH  - Male
MH  - Membrane Potentials
MH  - Microscopy, Fluorescence
MH  - Mitochondria/pathology
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*pathology
MH  - Staining and Labeling
MH  - T-Lymphocytes/cytology
EDAT- 2003/10/29 05:00
MHDA- 2004/02/10 05:00
CRDT- 2003/10/29 05:00
PHST- 2003/10/29 05:00 [pubmed]
PHST- 2004/02/10 05:00 [medline]
PHST- 2003/10/29 05:00 [entrez]
AID - 10.1002/cyto.b.10056 [doi]
PST - ppublish
SO  - Cytometry B Clin Cytom. 2003 Nov;56(1):16-22. doi: 10.1002/cyto.b.10056.