PMID- 14583148
OWN - NLM
STAT- MEDLINE
DCOM- 20040105
LR  - 20141120
IS  - 0882-8245 (Print)
IS  - 0882-8245 (Linking)
VI  - 16
IP  - 3
DP  - 2003
TI  - Parameters of humoral and cellular immunity following vaccination of pigs with a 
      European modified-live strain of porcine reproductive and respiratory syndrome 
      virus (PRRSV).
PG  - 335-46
AB  - Parameters of humoral and cellular immunity were investigated in pigs 
      experimentally infected with a modified-live European porcine reproductive and 
      respiratory syndrome virus (PRRSV, strain DV). PRRSV was detected by real-time 
      RT-PCR and PRRSV-specific antibodies by a commercial ELISA test-kit, 
      respectively. Interleukins IL-1alpha, IL-2, IL-4, IL-6, IL-8, IL-10, tumor 
      necrosis factor (TNF-alpha) and interferon-gamma (IFN-gamma) as well as IL-2 
      receptor (IL-2R) were quantified at mRNA level using RT-PCR. Subpopulations of 
      blood lymphocytes were assayed using flow cytometry. No significant changes 
      neither in cytokine expression nor in shifts of CD4 and CD8 markers could be 
      found, but similar curve diagrams concerning CD8 single positive T cells could be 
      observed in all vaccinated animals with an initial decrease and an increase 
      between post-infection days (PIDs) 7 and 14. In the vaccination group, TNF-alpha 
      and IL-6 tended to be increased at PIDs 22 and 40, whereas no increase could be 
      seen in IFN-gamma. When comparing the in vivo immune response to that being seen 
      in in vitro experiments, similar shifts of CD4/CD8 lymphocyte subpopulations may 
      be seen. Cytokine curve diagrams, however, do not reflect the in vitro findings 
      to that extent.
FAU - Sipos, Wolfgang
AU  - Sipos W
AD  - II Medical Clinic for Ruminants and Swine, University of Veterinary Medicine 
      Vienna, Vienna, Austria. wolfgang.sipos@vu-wein.ac.at
FAU - Duvigneau, Catharina
AU  - Duvigneau C
FAU - Pietschmann, Peter
AU  - Pietschmann P
FAU - Holler, Kurt
AU  - Holler K
FAU - Hartl, Romana
AU  - Hartl R
FAU - Wahl, Katharina
AU  - Wahl K
FAU - Steinborn, Ralf
AU  - Steinborn R
FAU - Gemeiner, Manfred
AU  - Gemeiner M
FAU - Willheim, Martin
AU  - Willheim M
FAU - Schmoll, Friedrich
AU  - Schmoll F
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Antibodies, Viral)
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/biosynthesis
MH  - Base Sequence
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cytokines/biosynthesis/genetics
MH  - DNA Primers/genetics
MH  - DNA, Viral/genetics
MH  - Female
MH  - Immunity, Cellular
MH  - In Vitro Techniques
MH  - Male
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - RNA, Messenger/genetics/metabolism
MH  - Sus scrofa
MH  - Vaccination
EDAT- 2003/10/30 05:00
MHDA- 2004/01/06 05:00
CRDT- 2003/10/30 05:00
PHST- 2003/10/30 05:00 [pubmed]
PHST- 2004/01/06 05:00 [medline]
PHST- 2003/10/30 05:00 [entrez]
AID - 10.1089/088282403322396136 [doi]
PST - ppublish
SO  - Viral Immunol. 2003;16(3):335-46. doi: 10.1089/088282403322396136.