PMID- 14584040
OWN - NLM
STAT- MEDLINE
DCOM- 20040120
LR  - 20071115
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 198
IP  - 1
DP  - 2004 Jan
TI  - Overexpression of thioredoxin reductase 1 regulates NF-kappa B activation.
PG  - 22-30
AB  - Thioredoxin reductase (TrxR) is a flavoprotein that contains a C-terminal 
      penultimate selenocysteine (Sec) and has an ability to reduce thioredoxin (Trx), 
      which regulates the activity of NF-kappa B. To date, three TrxR isozymes, TrxR1, 
      TrxR2, and TrxR3, have been identified. In the present study, we found that among 
      these isozymes only TrxR1 was induced by tumor necrosis factor-alpha (TNF alpha) 
      in vascular endothelial cells. Furthermore, the overexpression of TrxR1 enhanced 
      TNF alpha-induced DNA-binding activity of NF-kappa B and NF-kappa B-dependent 
      gene expression. The catalytic Sec residue of TrxR1, which is essential for 
      reducing Trx, was required for this NF-kappa B activation, and aurothiomalate, an 
      inhibitor of TrxR, suppressed TNF alpha-induced activation of NF-kappa B and the 
      expression of NF-kappa B-targeted proinflammatory genes such as E-selectin and 
      cyclooxygenase-2. These results suggest that TrxR1 may act as a positive 
      regulator of NF-kappa B and may play an important role in the cellular 
      inflammatory response.
FAU - Sakurai, Atsuko
AU  - Sakurai A
AD  - Department of Public Health and Molecular Toxicology, School of Pharmaceutical 
      Sciences, Kitasato University, Tokyo, Japan.
FAU - Yuasa, Kouji
AU  - Yuasa K
FAU - Shoji, Yasuko
AU  - Shoji Y
FAU - Himeno, Seiichiro
AU  - Himeno S
FAU - Tsujimoto, Masafumi
AU  - Tsujimoto M
FAU - Kunimoto, Manabu
AU  - Kunimoto M
FAU - Imura, Nobumasa
AU  - Imura N
FAU - Hara, Shuntaro
AU  - Hara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Antirheumatic Agents)
RN  - 0 (Isoenzymes)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 12244-57-4 (Gold Sodium Thiomalate)
RN  - EC 1.8.1.9 (TXNRD1 protein, human)
RN  - EC 1.8.1.9 (Thioredoxin Reductase 1)
RN  - EC 1.8.1.9 (Thioredoxin-Disulfide Reductase)
RN  - EC 1.8.1.9 (Txnrd1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Antirheumatic Agents/metabolism
MH  - Cattle
MH  - Cells, Cultured
MH  - Endothelium, Vascular/cytology/metabolism
MH  - Gene Expression Regulation
MH  - Gold Sodium Thiomalate/metabolism
MH  - Humans
MH  - Isoenzymes/genetics/*metabolism
MH  - Liver/metabolism
MH  - Mice
MH  - NF-kappa B/*metabolism
MH  - Thioredoxin Reductase 1
MH  - Thioredoxin-Disulfide Reductase/genetics/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2003/10/30 05:00
MHDA- 2004/01/21 05:00
CRDT- 2003/10/30 05:00
PHST- 2003/10/30 05:00 [pubmed]
PHST- 2004/01/21 05:00 [medline]
PHST- 2003/10/30 05:00 [entrez]
AID - 10.1002/jcp.10377 [doi]
PST - ppublish
SO  - J Cell Physiol. 2004 Jan;198(1):22-30. doi: 10.1002/jcp.10377.