PMID- 14585198
OWN - NLM
STAT- MEDLINE
DCOM- 20040604
LR  - 20061115
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 23
IP  - 10
DP  - 2003 Oct
TI  - Porcine TLR2 and TLR6: identification and their involvement in Mycoplasma 
      hyopneumoniae infection.
PG  - 583-90
AB  - We successfully cloned and sequenced porcine toll-like receptor (TLR2) and TLR6 
      cDNA from porcine alveolar macrophages stimulated with 10 microg/ml 
      lipopolysaccharide (LPS). The open reading frames (ORFs) of the porcine TLR2 and 
      TLR6 cDNA were shown to be 2358 and 2391 bp in length and to encode 785 and 796 
      amino acids, respectively. The predicted amino acid sequence of porcine TLR2 was 
      72.3% homologous to human TLR2 and 61.0% homologous to murine TLR2. That of 
      porcine TLR6 was 74.4% homologous to human TLR6 and 66.1% homologous to murine 
      TLR6. Porcine TLR2 and TLR6 genes were both mapped to porcine chromosome 8 (TLR2: 
      SSC8q21.1 --> 21.5; TLR6: SSC8p11.1 --> p21.1) by fluorescence in situ 
      hybridization (FISH) and radiation hybrid mapping. Western blot analysis 
      confirmed that TLR2 and TLR6 proteins were both expressed in porcine alveolar 
      macrophages. Further, antiporcine TLR2 and TLR6 antibodies synergistically 
      blocked tumor necrosis factor-alpha (TNF-alpha) production by porcine alveolar 
      macrophages stimulated with Mycoplasma hyopneumoniae. These results indicated 
      that both TLR2 and TLR6 are important in the recognition of M. hyopneumoniae in 
      porcine alveolar macrophages and will be useful in understanding innate immunity 
      against M. hyopneumoniae.
FAU - Muneta, Yoshihiro
AU  - Muneta Y
AD  - Department of Immunology, National Institute of Animal Health, Tsukuba, Ibaraki 
      305-0856, Japan. ymuneta@affrc.go.jp
FAU - Uenishi, Hirohide
AU  - Uenishi H
FAU - Kikuma, Reiko
AU  - Kikuma R
FAU - Yoshihara, Kazuhiro
AU  - Yoshihara K
FAU - Shimoji, Yoshihiro
AU  - Shimoji Y
FAU - Yamamoto, Ryuji
AU  - Yamamoto R
FAU - Hamashima, Noriyuki
AU  - Hamashima N
FAU - Yokomizo, Yuichi
AU  - Yokomizo Y
FAU - Mori, Yasuyuki
AU  - Mori Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Tlr6 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 6)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chromosomes, Mammalian/genetics
MH  - DNA, Complementary/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Macrophages, Alveolar/metabolism/virology
MH  - Male
MH  - Membrane Glycoproteins/antagonists & inhibitors/genetics/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Mycoplasma hyopneumoniae
MH  - Physical Chromosome Mapping
MH  - Pneumonia of Swine, Mycoplasmal/*immunology
MH  - Radiation Hybrid Mapping
MH  - Receptors, Cell Surface/antagonists & inhibitors/genetics/*metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - *Swine/genetics/immunology/microbiology
MH  - Toll-Like Receptor 2
MH  - Toll-Like Receptor 6
MH  - Toll-Like Receptors
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2003/10/31 05:00
MHDA- 2004/06/05 05:00
CRDT- 2003/10/31 05:00
PHST- 2003/10/31 05:00 [pubmed]
PHST- 2004/06/05 05:00 [medline]
PHST- 2003/10/31 05:00 [entrez]
AID - 10.1089/107999003322485080 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2003 Oct;23(10):583-90. doi: 
      10.1089/107999003322485080.