PMID- 14585673
OWN - NLM
STAT- MEDLINE
DCOM- 20040415
LR  - 20190728
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 21
IP  - 32
DP  - 2003 Dec 1
TI  - A DNA vaccine directed against a rainbow trout rhabdovirus induces early 
      protection against a nodavirus challenge in turbot.
PG  - 4661-7
AB  - A DNA vaccine encoding the envelope glycoprotein from a fish rhabdovirus, viral 
      hemorrhagic septicemia virus (VHSV), has previously been shown to induce both 
      early and long time protection against the virus in rainbow trout. Challenge 
      experiments have revealed that the immunity established shortly after vaccination 
      is cross-protective against heterologous fish rhabdoviruses. In this study, we 
      show that the DNA vaccine encoding the VHSV glycoprotein also induces early 
      protection against a non-enveloped, positive-sense RNA virus belonging to the 
      Nodavirus family, the Atlantic halibut nodavirus (AHNV). In a vaccine efficacy 
      test using juvenile turbot as model fish, the fish injected with the VHSV vaccine 
      were completely protected against a nodavirus challenge performed 8 days post 
      vaccination, while the cumulative mortality in the control group reached 54%. A 
      DNA vaccine carrying the gene encoding the capsid protein of AHNV revealed no 
      protective properties against the nodavirus challenge. Histological examination 
      of muscle tissue sections from the vaccine injection site showed that the DNA 
      vaccine against VHSV triggered a pronounced inflammatory response in turbot 
      similar to what has earlier been observed in rainbow trout.
FAU - Sommerset, Ingunn
AU  - Sommerset I
AD  - Institute of Marine Research, P.O. Box 1870 Nordnes, N-5817 Bergen, Norway. 
      ingunns@imr.no
FAU - Lorenzen, Ellen
AU  - Lorenzen E
FAU - Lorenzen, Niels
AU  - Lorenzen N
FAU - Bleie, Hogne
AU  - Bleie H
FAU - Nerland, Audun Helge
AU  - Nerland AH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Capsid Proteins)
RN  - 0 (Vaccines, DNA)
SB  - IM
MH  - Animals
MH  - Capsid Proteins/biosynthesis
MH  - Fish Diseases/immunology/*prevention & control/virology
MH  - Flatfishes/immunology/*virology
MH  - *Nodaviridae/immunology
MH  - Oncorhynchus mykiss/*virology
MH  - RNA Virus Infections/prevention & control/*veterinary/virology
MH  - Rhabdoviridae/*immunology
MH  - Species Specificity
MH  - Survival Analysis
MH  - Vaccination/methods/veterinary
MH  - Vaccines, DNA/*administration & dosage/immunology
EDAT- 2003/10/31 05:00
MHDA- 2004/04/16 05:00
CRDT- 2003/10/31 05:00
PHST- 2003/10/31 05:00 [pubmed]
PHST- 2004/04/16 05:00 [medline]
PHST- 2003/10/31 05:00 [entrez]
AID - S0264410X03005267 [pii]
AID - 10.1016/s0264-410x(03)00526-7 [doi]
PST - ppublish
SO  - Vaccine. 2003 Dec 1;21(32):4661-7. doi: 10.1016/s0264-410x(03)00526-7.