PMID- 14592428
OWN - NLM
STAT- MEDLINE
DCOM- 20040210
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 311
IP  - 2
DP  - 2003 Nov 14
TI  - Inhibition of hepatitis B virus replication by stably expressed shRNA.
PG  - 398-404
AB  - The major challenges for anti-hepatitis B Virus (HBV) therapy are the low 
      efficacy of current drugs and the occurrence of drug resistant HBV mutations. A 
      drug with new target sites or independent metabolic pathways may overcome these 
      shortcomings. Small interfering RNA (siRNA) offers the possibility of developing 
      a new anti-HBV therapy. Here we describe the almost complete inhibition of HBV 
      replication by stably expressed 21-mer short hairpin RNAs (shRNA). Besides the 
      conventional targets on HBV reverse-transcriptase, we also systemically targeted 
      other sites of pregenomic RNA, including direct repeat (DR) elements, S, core, 
      and X gene. Our results indicated that shRNAs can serve as efficient alternative 
      anti-HBV agents. They can also be used in combination with chemotherapy, because 
      they showed better effects on the inhibition of HBV replication due to different 
      mechanisms of drug actions.
FAU - Chen, Ying
AU  - Chen Y
AD  - The Institute of Molecular Biology and Open Laboratory of the Institute of 
      Molecular Technology for Drug Discovery and Synthesis, The University of Hong 
      Kong, Hong Kong, China.
FAU - Du, Dan
AU  - Du D
FAU - Wu, Jun
AU  - Wu J
FAU - Chan, Chun-Pong
AU  - Chan CP
FAU - Tan, Yuequi
AU  - Tan Y
FAU - Kung, Hsiang-fu
AU  - Kung HF
FAU - He, Ming-Liang
AU  - He ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (RNA, Small Interfering)
RN  - 2T8Q726O95 (Lamivudine)
SB  - IM
MH  - Carcinoma, Hepatocellular/*genetics/*virology
MH  - Cell Line, Tumor
MH  - Gene Expression Regulation, Viral/drug effects/*genetics
MH  - Genetic Therapy/methods
MH  - Hepatitis B virus/drug effects/*genetics/*growth & development
MH  - Humans
MH  - Lamivudine/pharmacology
MH  - RNA, Small Interfering/*genetics
MH  - *Virus Replication
EDAT- 2003/11/01 05:00
MHDA- 2004/02/11 05:00
CRDT- 2003/11/01 05:00
PHST- 2003/11/01 05:00 [pubmed]
PHST- 2004/02/11 05:00 [medline]
PHST- 2003/11/01 05:00 [entrez]
AID - S0006291X03020643 [pii]
AID - 10.1016/j.bbrc.2003.10.009 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2003 Nov 14;311(2):398-404. doi: 
      10.1016/j.bbrc.2003.10.009.