PMID- 14592439
OWN - NLM
STAT- MEDLINE
DCOM- 20040210
LR  - 20191210
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 311
IP  - 2
DP  - 2003 Nov 14
TI  - Establishment of Alb-DsRed2 transgenic rat for liver regeneration research.
PG  - 478-81
AB  - Because serum albumin is specifically produced by mature hepatocytes, detection 
      system of albumin producing cells could be a valuable tool to visualize liver 
      regeneration or development. We have developed here an albumin 
      enhancer/promoter-driven Alb-DsRed2 Tg rat that expresses DsRed2, having 
      liver-specific reporter gene expression of red fluorescent protein. To study the 
      transdifferentiation of bone marrow cells (BMCs) into albumin producing cells, 
      BMCs from the Alb-DsRed2 Tg rat were injected into rats having acute liver damage 
      caused by 2-acetylaminofluorene plus carbon tetrachloride and chronic liver 
      damage by repeated administration of CCl(4). DsRed2-positive cells were generated 
      in the recipient liver after BMC injection. The number of transdifferentiated 
      DsRed2-positive cells in chronic liver injury model was increased comparing with 
      that in acute injury model. We propose that the Alb-DsRed2 Tg rat is well suited 
      to studying in vivo liver regeneration.
FAU - Sato, Yuki
AU  - Sato Y
AD  - Division of Organ Replacement Research, Center for Molecular Medicine, Jichi 
      Medical School, 3311-1 Yakushiji, Minamikawachi, Kawachi, Tochigi, Japan.
FAU - Igarashi, Yuka
AU  - Igarashi Y
FAU - Hakamata, Yoji
AU  - Hakamata Y
FAU - Murakami, Takashi
AU  - Murakami T
FAU - Kaneko, Takashi
AU  - Kaneko T
FAU - Takahashi, Masafumi
AU  - Takahashi M
FAU - Seo, Norimasa
AU  - Seo N
FAU - Kobayashi, Eiji
AU  - Kobayashi E
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Luminescent Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Serum Albumin)
RN  - 0 (fluorescent protein 583)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - *Bone Marrow Transplantation
MH  - Cell Transplantation
MH  - Hepatitis, Chronic/genetics/*metabolism/surgery
MH  - Liver/*embryology/*metabolism/surgery
MH  - *Liver Regeneration
MH  - Luminescent Proteins/genetics/*metabolism
MH  - Male
MH  - *Models, Animal
MH  - Rats/genetics
MH  - Rats, Wistar
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Serum Albumin/genetics/*metabolism
EDAT- 2003/11/01 05:00
MHDA- 2004/02/11 05:00
CRDT- 2003/11/01 05:00
PHST- 2003/11/01 05:00 [pubmed]
PHST- 2004/02/11 05:00 [medline]
PHST- 2003/11/01 05:00 [entrez]
AID - S0006291X03020837 [pii]
AID - 10.1016/j.bbrc.2003.09.230 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2003 Nov 14;311(2):478-81. doi: 
      10.1016/j.bbrc.2003.09.230.