PMID- 14592830
OWN - NLM
STAT- MEDLINE
DCOM- 20040420
LR  - 20220224
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 103
IP  - 5
DP  - 2004 Mar 1
TI  - Inhibition of restenosis by tissue factor pathway inhibitor: in vivo and in vitro 
      evidence for suppressed monocyte chemoattraction and reduced gelatinolytic 
      activity.
PG  - 1653-61
AB  - Activation of inflammatory and procoagulant mechanisms is thought to contribute 
      significantly to the initiation of restenosis, a common complication after 
      balloon angioplasty of obstructed arteries. During this process, expression of 
      tissue factor (TF) represents one of the major physiologic triggers of 
      coagulation that results in thrombus formation and the generation of additional 
      signals leading to vascular smooth muscle cell (VSMC) proliferation and 
      migration. In this study, we have investigated the mechanisms by which inhibition 
      of coagulation at an early stage through overexpression of tissue factor pathway 
      inhibitor (TFPI), an endogenous inhibitor of TF, might reduce restenosis. In a 
      rabbit femoral artery model, percutaneous delivery of TFPI using a recombinant 
      adenoviral vector resulted in a significant reduction of the intimamedia ratio 21 
      days after injury. Investigating several markers of inflammation and coagulation, 
      we found reduced neointimal expression of monocyte chemoattractant protein-1 
      (MCP-1), lesional monocyte infiltration, and expression of vascular TF, matrix 
      metalloproteinase-2 (MMP-2), and MMP-9. Moreover, overexpression of TFPI 
      suppressed the autocrine release of platelet-derived growth factor BB (PDGF-BB), 
      MCP-1, and MMP-2 in response to factors VIIa and Xa from VSMCs in vitro and 
      inhibited monocyte TF activity. These results suggest that TFPI exerts its action 
      in vivo through not only thrombotic, but also nonthrombotic mechanisms.
FAU - Kopp, Christoph W
AU  - Kopp CW
AD  - 2nd Department of Medicine, Division of Angiology, University of Vienna Medical 
      School, Vienna, Austria. christoph.kopp@univie.ac.at
FAU - Holzenbein, Thomas
AU  - Holzenbein T
FAU - Steiner, Sabine
AU  - Steiner S
FAU - Marculescu, Rodrig
AU  - Marculescu R
FAU - Bergmeister, Helga
AU  - Bergmeister H
FAU - Seidinger, Daniela
AU  - Seidinger D
FAU - Mosberger, Isabella
AU  - Mosberger I
FAU - Kaun, Christoph
AU  - Kaun C
FAU - Cejna, Manfred
AU  - Cejna M
FAU - Horvat, Reinhard
AU  - Horvat R
FAU - Wojta, Johann
AU  - Wojta J
FAU - Maurer, Gerald
AU  - Maurer G
FAU - Binder, Bernd R
AU  - Binder BR
FAU - Breuss, Johannes M
AU  - Breuss JM
FAU - Ecker, Rupert C
AU  - Ecker RC
FAU - de Martin, Rainer
AU  - de Martin R
FAU - Minar, Erich
AU  - Minar E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031030
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA, Complementary)
RN  - 0 (Lipoproteins)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 0 (lipoprotein-associated coagulation inhibitor)
RN  - 1B56C968OA (Becaplermin)
RN  - 9035-58-9 (Thromboplastin)
RN  - EC 3.4.21.21 (Factor VIIa)
RN  - EC 3.4.21.6 (Factor Xa)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Angioplasty
MH  - Animals
MH  - Becaplermin
MH  - Cell Division
MH  - Cell Movement
MH  - Chemokine CCL2/biosynthesis/metabolism
MH  - Cloning, Molecular
MH  - Constriction, Pathologic
MH  - DNA, Complementary/metabolism
MH  - Factor VIIa/metabolism
MH  - Factor Xa/metabolism
MH  - *Graft Occlusion, Vascular
MH  - Humans
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Inflammation
MH  - Lipoproteins/pharmacology
MH  - Matrix Metalloproteinase 2/biosynthesis/metabolism
MH  - Microscopy, Confocal
MH  - Monocytes/*cytology/metabolism
MH  - Muscle, Smooth, Vascular/cytology
MH  - Myocytes, Smooth Muscle/cytology
MH  - Platelet-Derived Growth Factor/biosynthesis/metabolism
MH  - Precipitin Tests
MH  - Proto-Oncogene Proteins c-sis
MH  - Rabbits
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Thromboplastin/*antagonists & inhibitors
MH  - Time Factors
MH  - Transfection
MH  - Transgenes
MH  - U937 Cells
EDAT- 2003/11/01 05:00
MHDA- 2004/04/21 05:00
CRDT- 2003/11/01 05:00
PHST- 2003/11/01 05:00 [pubmed]
PHST- 2004/04/21 05:00 [medline]
PHST- 2003/11/01 05:00 [entrez]
AID - S0006-4971(20)50075-X [pii]
AID - 10.1182/blood-2003-04-1148 [doi]
PST - ppublish
SO  - Blood. 2004 Mar 1;103(5):1653-61. doi: 10.1182/blood-2003-04-1148. Epub 2003 Oct 
      30.