PMID- 14593184 OWN - NLM STAT- MEDLINE DCOM- 20031208 LR - 20220318 IS - 1095-9203 (Electronic) IS - 0036-8075 (Linking) VI - 302 IP - 5646 DP - 2003 Oct 31 TI - DNA methylation-related chromatin remodeling in activity-dependent BDNF gene regulation. PG - 890-3 AB - In conjunction with histone modifications, DNA methylation plays critical roles in gene silencing through chromatin remodeling. Changes in DNA methylation perturb neuronal function, and mutations in a methyl-CpG-binding protein, MeCP2, are associated with Rett syndrome. We report that increased synthesis of brain-derived neurotrophic factor (BDNF) in neurons after depolarization correlates with a decrease in CpG methylation within the regulatory region of the Bdnf gene. Moreover, increased Bdnf transcription involves dissociation of the MeCP2-histone deacetylase-mSin3A repression complex from its promoter. Our findings suggest that DNA methylation-related chromatin remodeling is important for activity-dependent gene regulation that may be critical for neural plasticity. FAU - Martinowich, Keri AU - Martinowich K AD - Neuroscience Interdepartmental Program, UCLA School of Medicine, 760 Westwood Plaza, Los Angeles, CA 90095, USA. FAU - Hattori, Daisuke AU - Hattori D FAU - Wu, Hao AU - Wu H FAU - Fouse, Shaun AU - Fouse S FAU - He, Fei AU - He F FAU - Hu, Yan AU - Hu Y FAU - Fan, Guoping AU - Fan G FAU - Sun, Yi E AU - Sun YE LA - eng GR - NS44405/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Science JT - Science (New York, N.Y.) JID - 0404511 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Chromatin) RN - 0 (Chromosomal Proteins, Non-Histone) RN - 0 (Cyclic AMP Response Element-Binding Protein) RN - 0 (DNA-Binding Proteins) RN - 0 (Mecp2 protein, mouse) RN - 0 (Methyl-CpG-Binding Protein 2) RN - 0 (Repressor Proteins) RN - 0 (SIN3A transcription factor) RN - 0 (Transcription Factors) RN - 660YQ98I10 (Potassium Chloride) RN - EC 3.5.1.98 (Histone Deacetylases) RN - EC 3.5.1.98 (Sin3 Histone Deacetylase and Corepressor Complex) SB - IM CIN - Science. 2003 Oct 31;302(5646):793-5. PMID: 14593157 MH - Animals MH - Brain-Derived Neurotrophic Factor/biosynthesis/*genetics MH - Cells, Cultured MH - Chromatin/*metabolism MH - *Chromosomal Proteins, Non-Histone MH - CpG Islands/*physiology MH - Cyclic AMP Response Element-Binding Protein/metabolism MH - *DNA Methylation MH - DNA-Binding Proteins/metabolism MH - *Gene Expression Regulation MH - Gene Silencing MH - Histone Deacetylases/metabolism MH - Methyl-CpG-Binding Protein 2 MH - Mice MH - Mice, Inbred BALB C MH - Models, Genetic MH - Neuronal Plasticity MH - Neurons/*metabolism/physiology MH - Potassium Chloride/pharmacology MH - Promoter Regions, Genetic MH - Repressor Proteins/metabolism MH - Response Elements MH - Sin3 Histone Deacetylase and Corepressor Complex MH - Transcription Factors/metabolism MH - Transcription, Genetic MH - Transfection EDAT- 2003/11/01 05:00 MHDA- 2003/12/10 05:00 CRDT- 2003/11/01 05:00 PHST- 2003/11/01 05:00 [pubmed] PHST- 2003/12/10 05:00 [medline] PHST- 2003/11/01 05:00 [entrez] AID - 302/5646/890 [pii] AID - 10.1126/science.1090842 [doi] PST - ppublish SO - Science. 2003 Oct 31;302(5646):890-3. doi: 10.1126/science.1090842.