PMID- 14593234
OWN - NLM
STAT- MEDLINE
DCOM- 20040618
LR  - 20171101
IS  - 0014-312X (Print)
IS  - 0014-312X (Linking)
VI  - 35
IP  - 6
DP  - 2003 Nov-Dec
TI  - Persistent extracellular matrix remodelling at the interface to polymers used for 
      hernia repair.
PG  - 497-504
AB  - On the one hand, recurrence rates and postoperative complications following 
      hernia repair are supposed to be influenced by the kind of mesh material used. On 
      the other hand, an impaired collagen metabolism and cleavage within connective 
      tissue has been suggested as decisive factor in the pathogenesis of recurrent 
      hernia formation. The aim of our study was, therefore, to analyze the impact of 
      commonly used mesh materials on quality of collagen deposition, expression of 
      collagenases (matrix metalloproteinases; MMP-1/MMP-13), and specific tissue 
      inhibitors of MMPs (TIMPs) in an animal study. Four different mesh materials were 
      used (Prolene = polypropylene, Mersilene = polyester, and Vypro and Vypro II = 
      combinations of polypropylene and polyglactin) and implanted as abdominal wall 
      replacement in 60 male Wistar rats. Mesh samples were explanted after 3, 21, and 
      90 days and investigated using immunohistochemistry (expression of MMP-1/MMP-13 
      and TIMP-1) and cross-polarization microscopy (percentage of collagen type III to 
      overall collagen). Besides an insufficient collagen composition with an increased 
      percentage of collagen type III, we found a complex expression of collagenases 
      and their inhibitors combined with a persistent chronic foreign-body reaction 
      even 90 days after implantation. Except for TIMP-1 expression, which was 
      significantly related to a lowered amount of inflammatory (r = -0.980, p = 0.02) 
      and connective tissue formation (r = -0.951, p = 0.049), there was no relation to 
      the expression of collagenases (MMP-1/MMP-13) with regard to the amount of 
      inflammatory and connective tissue formation despite partly significant 
      differences between implanted polymers.
CI  - Copyright 2003 S. Karger AG, Basel
FAU - Junge, K
AU  - Junge K
AD  - Department of Surgery, German Centre of Excellence for Biomaterial and Implant 
      Pathology, Technical University of Aachen, Aachen, Germany. 
      karsten.junge@post.rwth-aachen.de
FAU - Rosch, R
AU  - Rosch R
FAU - Bialasinski, L
AU  - Bialasinski L
FAU - Klinge, U
AU  - Klinge U
FAU - Klosterhalfen, B
AU  - Klosterhalfen B
FAU - Schumpelick, V
AU  - Schumpelick V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Eur Surg Res
JT  - European surgical research. Europaische chirurgische Forschung. Recherches 
      chirurgicales europeennes
JID - 0174752
RN  - 0 (Collagen Type III)
RN  - 0 (Polyesters)
RN  - 0 (Polypropylenes)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 34346-01-5 (Polyglactin 910)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
RN  - EC 3.4.24.- (Mmp13 protein, rat)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Animals
MH  - Collagen Type III/metabolism
MH  - Collagenases/metabolism
MH  - Extracellular Matrix/*metabolism
MH  - Hernia, Ventral/*surgery
MH  - Immunohistochemistry
MH  - Male
MH  - Matrix Metalloproteinase 1/metabolism
MH  - Matrix Metalloproteinase 13
MH  - Polyesters/pharmacology
MH  - Polyglactin 910/pharmacology
MH  - Polypropylenes/pharmacology
MH  - Postoperative Complications/metabolism/prevention & control
MH  - Rats
MH  - Rats, Wistar
MH  - *Surgical Mesh
MH  - Tissue Inhibitor of Metalloproteinase-1/metabolism
MH  - Wound Healing
EDAT- 2003/11/01 05:00
MHDA- 2004/06/24 05:00
CRDT- 2003/11/01 05:00
PHST- 2003/03/24 00:00 [received]
PHST- 2003/06/02 00:00 [accepted]
PHST- 2003/11/01 05:00 [pubmed]
PHST- 2004/06/24 05:00 [medline]
PHST- 2003/11/01 05:00 [entrez]
AID - 73389 [pii]
AID - 10.1159/000073389 [doi]
PST - ppublish
SO  - Eur Surg Res. 2003 Nov-Dec;35(6):497-504. doi: 10.1159/000073389.