PMID- 1459374
OWN - NLM
STAT- MEDLINE
DCOM- 19930113
LR  - 20190907
IS  - 0272-0590 (Print)
IS  - 0272-0590 (Linking)
VI  - 19
IP  - 3
DP  - 1992 Oct
TI  - Examination of the local lymph node assay for use in contact sensitization risk 
      assessment.
PG  - 438-45
AB  - The purpose of this study was to evaluate the utility of the murine local lymph 
      node assay (LLNA) for contact sensitization risk assessment. Cellular 
      proliferative activity in draining lymph nodes was determined for individual 
      animals on Day 5 following four daily epicutaneous applications of the test 
      chemical to the ears. Seventeen chemicals were tested, covering a range of 
      materials including preservatives, drug actives, and perfume raw materials. The 
      assay was found to be useful for identifying strong, moderate, and some weak 
      sensitizers as defined by other testing methods (guinea pig, human). For 
      evaluating the antigen specificity of the LLNA proliferative response, an in 
      vitro blastogenesis assay was used. Dendritic cells (DC) isolated from lymph 
      nodes of mice treated 24 hr earlier with trinitrochlorobenzene (TNCB) were 
      capable of in vitro stimulation of lymphocytes from TNCB-sensitized mice, but not 
      lymphocytes from mice sensitized to the preservative mixture of 
      5-chloro-2-methylisothiazolinone plus 2-methylisothiazolinone (MCI/MI). 
      Conversely, DC from mice treated 24 hr earlier with MCI/MI were capable of 
      stimulating lymphocytes from MCI/MI-sensitized mice, but were unable to stimulate 
      lymphocytes from TNCB-sensitized mice, demonstrating the specificity of the 
      response. The results of these studies support the use of the murine LLNA for 
      both investigative and predictive contact sensitization testing. The LLNA offers 
      the advantages of requiring less time for completion, incorporating an objective 
      endpoint, requiring approximately half the number of animals, and being less 
      costly than most currently employed guinea pig test methods. In addition, we 
      believe the murine LLNA is a useful test to incorporate into a scheme for contact 
      sensitization risk assessment.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Gerberick, G F
AU  - Gerberick GF
AD  - Procter and Gamble Company, Miami Valley Laboratories, Cincinnati, Ohio.
FAU - House, R V
AU  - House RV
FAU - Fletcher, E R
AU  - Fletcher ER
FAU - Ryan, C A
AU  - Ryan CA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Fundam Appl Toxicol
JT  - Fundamental and applied toxicology : official journal of the Society of 
      Toxicology
JID - 8200838
SB  - IM
MH  - Animals
MH  - Dendrites/drug effects
MH  - Dermatitis, Contact/*pathology
MH  - Female
MH  - Guinea Pigs
MH  - Humans
MH  - Lymph Nodes/drug effects/*physiology
MH  - Lymphocyte Activation/*drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred CBA
MH  - Risk Factors
EDAT- 1992/10/01 00:00
MHDA- 1992/10/01 00:01
CRDT- 1992/10/01 00:00
PHST- 1992/10/01 00:00 [pubmed]
PHST- 1992/10/01 00:01 [medline]
PHST- 1992/10/01 00:00 [entrez]
AID - 10.1016/0272-0590(92)90183-i [doi]
PST - ppublish
SO  - Fundam Appl Toxicol. 1992 Oct;19(3):438-45. doi: 10.1016/0272-0590(92)90183-i.