PMID- 1459458
OWN - NLM
STAT- MEDLINE
DCOM- 19930113
LR  - 20240109
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 6
IP  - 12A
DP  - 1992 Dec
TI  - Serine-to-alanine substitutions at the amino-terminal region of phytochrome A 
      result in an increase in biological activity.
PG  - 2364-72
AB  - We have used a tobacco transgenic plant system to assay the structure/function 
      relationship of phytochrome A (phyA), a plant photoreceptor. The amino terminus 
      of phyA from different plant species is very rich in serine residues. To 
      investigate whether these serine residues are required for phytochrome function, 
      the first 10 serine codons encoding amino acid residues 2-4, 10-14, 19, and 20 in 
      the amino-terminal domain of the rice phyA gene (phyA) were changed to alanine 
      codons. The mutant (S/A phyA), as well as the wild-type phyA cDNA, was placed 
      under the control of the 35S promoter, and the chimeric genes were transferred 
      into the tobacco genome by Agrobacterium-mediated transformation. Transgenic 
      tobacco plants expressing either wild-type or S/A phyA showed similar phenotypic 
      alterations, including dwarfism and dark-green leaves. However, hypocotyl 
      elongation experiments revealed that transgenic seedlings expressing S/A phyA 
      showed a higher amplitude of the red light response with respect to the 
      inhibition of hypocotyl elongation. The observed difference is not correlated 
      with expression levels of the transgene. The chromophore is attached to the 
      mutant phyA apoprotein (PHY A), and the mutant photoreceptor is photoreversible, 
      giving a difference spectrum indistinguishable from that of the rice phyA. Our 
      results indicate that the S/A mutant has a higher biological activity as compared 
      with the wild-type rice phyA.
FAU - Stockhaus, J
AU  - Stockhaus J
AD  - Rockefeller University, Laboratory of Plant Molecular Biology, New York, New York 
      10021-6399.
FAU - Nagatani, A
AU  - Nagatani A
FAU - Halfter, U
AU  - Halfter U
FAU - Kay, S
AU  - Kay S
FAU - Furuya, M
AU  - Furuya M
FAU - Chua, N H
AU  - Chua NH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 11121-56-5 (Phytochrome)
RN  - 452VLY9402 (Serine)
RN  - OF5P57N2ZX (Alanine)
SB  - IM
GS  - phyA
MH  - Alanine/genetics/*metabolism
MH  - Amino Acid Sequence
MH  - Chromatography, Gel
MH  - Enzyme Stability
MH  - Immunoblotting
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Oryza
MH  - Phytochrome/genetics/*metabolism
MH  - Plants, Genetically Modified
MH  - Plants, Toxic
MH  - Serine/genetics/*metabolism
MH  - Structure-Activity Relationship
MH  - Nicotiana
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
AID - 10.1101/gad.6.12a.2364 [doi]
PST - ppublish
SO  - Genes Dev. 1992 Dec;6(12A):2364-72. doi: 10.1101/gad.6.12a.2364.