PMID- 14595335
OWN - NLM
STAT- MEDLINE
DCOM- 20040116
LR  - 20220129
IS  - 1465-7392 (Print)
IS  - 1465-7392 (Linking)
VI  - 5
IP  - 12
DP  - 2003 Dec
TI  - Citron-N is a neuronal Rho-associated protein involved in Golgi organization 
      through actin cytoskeleton regulation.
PG  - 1071-8
AB  - The actin cytoskeleton is best known for its role during cellular morphogenesis. 
      However, other evidence suggests that actin is also crucial for the organization 
      and dynamics of membrane organelles such as endosomes and the Golgi complex. As 
      in morphogenesis, the Rho family of small GTPases are key mediators of organelle 
      actin-driven events, although it is unclear how these ubiquitously distributed 
      proteins are activated to regulate actin dynamics in an organelle-specific 
      manner. Here we show that the brain-specific Rho-binding protein Citron-N is 
      enriched at, and associates with, the Golgi apparatus of hippocampal neurons in 
      culture. Suppression of the whole protein or expression of a mutant form lacking 
      the Rho-binding activity results in dispersion of the Golgi apparatus. In 
      contrast, high intracellular levels induce localized accumulation of RhoA and 
      filamentous actin, protecting the Golgi from the rupture normally produced by 
      actin depolymerization. Biochemical and functional analyses indicate that 
      Citron-N controls actin locally by assembling together the Rho effector ROCK-II 
      and the actin-binding, neuron-specific, protein Profilin-IIa (PIIa). Together 
      with recent data on endosomal dynamics, our results highlight the importance of 
      organelle-specific Rho modulators for actin-dependent organelle organization and 
      dynamics.
FAU - Camera, Paola
AU  - Camera P
AD  - Dipartimento di Genetica, Biologia e Biochimica, Universita Degli Studi di Torino 
      Via Santena, 5 bis, Torino, Italy.
FAU - da Silva, Jorge Santos
AU  - da Silva JS
FAU - Griffiths, Gareth
AU  - Griffiths G
FAU - Giuffrida, Maria Gabriella
AU  - Giuffrida MG
FAU - Ferrara, Luciana
AU  - Ferrara L
FAU - Schubert, Vanessa
AU  - Schubert V
FAU - Imarisio, Sara
AU  - Imarisio S
FAU - Silengo, Lorenzo
AU  - Silengo L
FAU - Dotti, Carlos G
AU  - Dotti CG
FAU - Di Cunto, Ferdinando
AU  - Di Cunto F
LA  - eng
GR  - GGP02245/TI_/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031102
PL  - England
TA  - Nat Cell Biol
JT  - Nature cell biology
JID - 100890575
RN  - 0 (Actins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Contractile Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Pfn1 protein, rat)
RN  - 0 (Profilins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Proteins)
RN  - EC 2.7.1.- (citron-kinase)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - Actin Cytoskeleton/*metabolism
MH  - Actins/metabolism
MH  - Animals
MH  - Binding Sites/genetics
MH  - *Cell Cycle Proteins
MH  - Cell Differentiation/*physiology
MH  - Cells, Cultured
MH  - *Contractile Proteins
MH  - Fetus
MH  - Golgi Apparatus/*metabolism
MH  - Hippocampus/metabolism
MH  - Intracellular Signaling Peptides and Proteins
MH  - Microfilament Proteins/metabolism
MH  - Neurons/*metabolism/ultrastructure
MH  - Profilins
MH  - Protein Binding/genetics
MH  - Protein Isoforms/genetics/metabolism
MH  - Protein Serine-Threonine Kinases/metabolism
MH  - Proteins/genetics/*metabolism
MH  - Rats
MH  - rho GTP-Binding Proteins/*metabolism
MH  - rho-Associated Kinases
MH  - rhoA GTP-Binding Protein/metabolism
EDAT- 2003/11/05 05:00
MHDA- 2004/01/17 05:00
CRDT- 2003/11/05 05:00
PHST- 2003/03/20 00:00 [received]
PHST- 2003/09/01 00:00 [accepted]
PHST- 2003/11/05 05:00 [pubmed]
PHST- 2004/01/17 05:00 [medline]
PHST- 2003/11/05 05:00 [entrez]
AID - ncb1064 [pii]
AID - 10.1038/ncb1064 [doi]
PST - ppublish
SO  - Nat Cell Biol. 2003 Dec;5(12):1071-8. doi: 10.1038/ncb1064. Epub 2003 Nov 2.