PMID- 14595754
OWN - NLM
STAT- MEDLINE
DCOM- 20040105
LR  - 20171116
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 201
IP  - 3
DP  - 2003 Nov
TI  - Chromosomal imbalances: a hallmark of tumour relapse in primary cutaneous CD30+ 
      T-cell lymphoma.
PG  - 421-9
AB  - Primary cutaneous CD30+ large T-cell lymphoma (CD30+ CTCL) is a subset of 
      non-epidermotropic primary cutaneous T-cell lymphoma. Although frequent 
      spontaneous regression may be observed, skin relapses occur frequently. 
      Cytogenetic abnormalities that could play a role in CD30+ CTCL tumour 
      pathogenesis and relapses remain unknown. The identification of recurrent 
      cytogenetic abnormalities is hampered by difficulty in culturing tumours and the 
      lack of CD30+ CTCL serial studies comparing genetic changes both at diagnosis and 
      at relapse. The purpose of this study was to investigate the cytogenetic 
      abnormalities present in a series of 13 CD30+ CTCL samples obtained from nine 
      patients fulfilling both EORTC and WHO diagnostic criteria, by the use of 
      comparative genomic hybridization (CGH). CGH analysis revealed a non-random 
      distribution of genetic imbalances between relapsing and non-relapsing disease. 
      In relapsing disease, chromosomal abnormalities were detected both in the primary 
      tumour and in relapses. The mean number of changes in non-relapsing disease was 
      0.33 (range 0-1), compared with 6.29 (range 1-16) in relapsing disease. The 
      recurrent chromosomes involved in relapsing disease were chromosomes 6 (86%), 9 
      (86%), and 18 (43%). While chromosome 9 was mostly affected by gain, chromosomes 
      6 and 18 mainly contained regions of loss, exclusively on 6q and 18p. The common 
      regions of deletion were 6q21 and 18p11.3. In one patient, we successfully 
      cultured tumour cells from a skin biopsy from a second relapse. The G-banded 
      karyotype was concordant with both CGH and fluorescence in situ hybridization 
      (FISH) results. Although further studies are required to strengthen these data, 
      this CGH analysis demonstrates chromosomal imbalances that may be involved in the 
      pathogenesis of relapsing CD30+ CTCL.
CI  - Copyright 2003 John Wiley & Sons, Ltd.
FAU - Prochazkova, Martina
AU  - Prochazkova M
AD  - Histology and Molecular Pathology Laboratory, EA2406, V Segalen University, 
      Bordeaux, France.
FAU - Chevret, Edith
AU  - Chevret E
FAU - Beylot-Barry, Marie
AU  - Beylot-Barry M
FAU - Sobotka, Jiri
AU  - Sobotka J
FAU - Vergier, Beatrice
AU  - Vergier B
FAU - Delaunay, Michele
AU  - Delaunay M
FAU - Turmo, Michele
AU  - Turmo M
FAU - Ferrer, Jacky
AU  - Ferrer J
FAU - Kuglik, Petr
AU  - Kuglik P
FAU - Merlio, Jean-Philippe
AU  - Merlio JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Ki-1 Antigen)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 18/genetics
MH  - Chromosomes, Human, Pair 6/genetics
MH  - Chromosomes, Human, Pair 9/genetics
MH  - DNA, Neoplasm/analysis
MH  - Gene Deletion
MH  - Gene Rearrangement
MH  - Humans
MH  - Hybridization, Genetic/genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping/methods
MH  - Ki-1 Antigen/*genetics
MH  - Lymphoma, Large B-Cell, Diffuse/*genetics
MH  - Lymphoma, T-Cell, Cutaneous/*genetics
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local/genetics
MH  - Skin Neoplasms/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2003/11/05 05:00
MHDA- 2004/01/06 05:00
CRDT- 2003/11/05 05:00
PHST- 2003/11/05 05:00 [pubmed]
PHST- 2004/01/06 05:00 [medline]
PHST- 2003/11/05 05:00 [entrez]
AID - 10.1002/path.1469 [doi]
PST - ppublish
SO  - J Pathol. 2003 Nov;201(3):421-9. doi: 10.1002/path.1469.