PMID- 14596667
OWN - NLM
STAT- MEDLINE
DCOM- 20040127
LR  - 20190709
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 179
IP  - 2
DP  - 2003 Nov
TI  - N-methyl-D-aspartate receptor activity and estradiol: separate regulation of cell 
      proliferation in the dentate gyrus of adult female meadow vole.
PG  - 155-63
AB  - We have previously found that estradiol increases (within 4 h) but then decreases 
      (within 48 h) cell proliferation in the dentate gyrus of adult female 
      ovariectomized (OVX) rats and of intact meadow voles and that estradiol partially 
      stimulates adrenal activity to suppress cell proliferation in rats. Estradiol 
      enhances N-methyl-D-aspartate receptor (NMDAr) activity and NMDAr activation 
      suppresses cell proliferation in the adult rodent dentate gyrus. Therefore, we 
      tested whether estradiol alters cell proliferation in the dentate gyrus of adult 
      OVX female meadow voles by stimulating NMDAr activity. In experiment 1, OVX 
      females were injected with estradiol (10 micro g) or oil and then with NMDA (30 
      mg/kg) or vehicle 3 h later and bromodeoxyuridine 4 h later (BrdU; 50 mg/kg). 
      Voles were perfused 1 h after BrdU injection. Relative to oil vehicle, estradiol 
      increased (P</=0.001) and NMDA decreased (P</=0.006) labeled cell number. 
      Coadministration of estradiol/NMDA increased labeled cell numbers relative to 
      NMDA alone (P</=0.03), suggesting that within 4 h estradiol does not influence 
      the effect of NMDA receptors on cell proliferation. In experiment 2, OVX females 
      were injected with either estradiol or oil and then with either MK-801 (1 mg/kg) 
      or vehicle 47 h later and BrdU 48 h later. The animals were perfused 1 h after 
      BrdU was injected. Relative to oil-treated voles, estradiol-treated voles had 
      fewer (P<0.006) and MK-801-treated voles had more labeled cells (P</=0.0001) in 
      the dentate gyrus. However, estradiol did not appear to stimulate NMDA receptors 
      to suppress cell proliferation because estradiol (48 h)/MK-801-treated voles had 
      fewer BrdU-labeled cells than oil (48 h)/MK-801-treated voles (P</=0.06). The 
      results show that estradiol time-dependently influences cell proliferation but 
      that estradiol does not stimulate NMDAr activity to influence cell proliferation 
      in the dentate gyrus of adult voles.
FAU - Ormerod, B K
AU  - Ormerod BK
AD  - Department of Psychology and Graduate Neuroscience Program, 2136 West Mall, The 
      University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4.
FAU - Falconer, E M
AU  - Falconer EM
FAU - Galea, L A M
AU  - Galea LA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Coloring Agents)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 4TI98Z838E (Estradiol)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 6LR8C1B66Q (Dizocilpine Maleate)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Arvicolinae
MH  - Bromodeoxyuridine/pharmacology
MH  - Cell Count
MH  - Cell Division/drug effects
MH  - Coloring Agents
MH  - Dentate Gyrus/*cytology/metabolism
MH  - Dizocilpine Maleate/pharmacology
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Immunohistochemistry/methods
MH  - N-Methylaspartate/antagonists & inhibitors/pharmacology
MH  - Neuroprotective Agents/pharmacology
MH  - Ovariectomy
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Time Factors
EDAT- 2003/11/05 05:00
MHDA- 2004/01/28 05:00
CRDT- 2003/11/05 05:00
PHST- 2003/11/05 05:00 [pubmed]
PHST- 2004/01/28 05:00 [medline]
PHST- 2003/11/05 05:00 [entrez]
AID - 10.1677/joe.0.1790155 [doi]
PST - ppublish
SO  - J Endocrinol. 2003 Nov;179(2):155-63. doi: 10.1677/joe.0.1790155.