PMID- 14596916
OWN - NLM
STAT- MEDLINE
DCOM- 20031223
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 554
IP  - 1-2
DP  - 2003 Nov 6
TI  - Methylation at CpG islands in intron 1 of EGR2 confers enhancer-like activity.
PG  - 67-72
AB  - We previously demonstrated several lines of evidence indicating that early growth 
      response 2 (EGR2) functions as a tumor suppressor, partly on the basis that its 
      expression was often decreased in human tumors and cancer cell lines. Here we 
      report a possible molecular mechanism to account for down-regulation of EGR2 in 
      tumor cells. Although no genetic mutations in the gene or alterations in 
      methylation status of its promoter were detected, we found a high degree of 
      methylation at CpG islands in intron 1 of EGR2 in cell lines that were expressing 
      this gene at a high level. Moreover, reporter gene experiments revealed that 
      methylated intron 1 had somehow conferred enhancer-like activity. The data imply 
      the existence of a previously unsuspected mechanism of gene expression 
      regulation.
FAU - Unoki, Motoko
AU  - Unoki M
AD  - Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical 
      Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, 
      Japan.
FAU - Nakamura, Yusuke
AU  - Nakamura Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (EGR2 protein, human)
RN  - 0 (Early Growth Response Protein 2)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Base Sequence
MH  - Cell Line, Tumor
MH  - CpG Islands/*physiology
MH  - *DNA Methylation
MH  - DNA-Binding Proteins/*genetics
MH  - Down-Regulation
MH  - Early Growth Response Protein 2
MH  - Enhancer Elements, Genetic/*genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Genes, Reporter
MH  - Humans
MH  - Introns/*genetics
MH  - Molecular Sequence Data
MH  - Sequence Analysis, DNA/methods
MH  - Transcription Factors/*genetics
EDAT- 2003/11/05 05:00
MHDA- 2003/12/24 05:00
CRDT- 2003/11/05 05:00
PHST- 2003/11/05 05:00 [pubmed]
PHST- 2003/12/24 05:00 [medline]
PHST- 2003/11/05 05:00 [entrez]
AID - S0014579303010925 [pii]
AID - 10.1016/s0014-5793(03)01092-5 [doi]
PST - ppublish
SO  - FEBS Lett. 2003 Nov 6;554(1-2):67-72. doi: 10.1016/s0014-5793(03)01092-5.