PMID- 14597634
OWN - NLM
STAT- MEDLINE
DCOM- 20040423
LR  - 20220311
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 279
IP  - 4
DP  - 2004 Jan 23
TI  - Cytosolic peroxiredoxin attenuates the activation of Jnk and p38 but potentiates 
      that of Erk in Hela cells stimulated with tumor necrosis factor-alpha.
PG  - 2535-43
AB  - Tumor necrosis factor-alpha (TNF-alpha) induces the activation of all three types 
      of mitogen-activated protein kinase (MAPK): c-Jun NH(2)-terminal kinase (JNK), 
      p38, and extracellular signal-regulated kinase (ERK). This cytokine also induces 
      the production of several types of reactive oxygen species, including H(2)O(2). 
      With the use both of HeLa cells expressing wild-type or dominant negative forms 
      of the cytosolic peroxidase peroxiredoxin II and of mouse embryonic fibroblasts 
      deficient in this protein, we evaluated the roles of H(2)O(2) in the activation 
      of MAPKs by TNF-alpha. In vitro kinase assays as well as immunoblot analysis with 
      antibodies specific for activated MAPKs indicated that H(2)O(2) produced in 
      response to TNF-alpha potentiates the activation of JNK and p38 induced by this 
      cytokine but inhibits that of ERK. Our results also suggest that cytosolic 
      peroxiredoxins are important regulators of TNF signaling pathways.
FAU - Kang, Sang Won
AU  - Kang SW
AD  - Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, 
      Maryland 20892, USA.
FAU - Chang, Tong-Shin
AU  - Chang TS
FAU - Lee, Tae-Hoon
AU  - Lee TH
FAU - Kim, Eun Seon
AU  - Kim ES
FAU - Yu, Dae-Yeul
AU  - Yu DY
FAU - Rhee, Sue Goo
AU  - Rhee SG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031103
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 1.11.1.15 (Peroxiredoxins)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - Cytosol/metabolism
MH  - Enzyme Activation/drug effects
MH  - HeLa Cells
MH  - Humans
MH  - *JNK Mitogen-Activated Protein Kinases
MH  - MAP Kinase Kinase 4
MH  - Mitogen-Activated Protein Kinase Kinases/*metabolism
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Peroxidases/*metabolism
MH  - Peroxiredoxins
MH  - Signal Transduction/drug effects
MH  - Tumor Necrosis Factor-alpha/*metabolism
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2003/11/05 05:00
MHDA- 2004/04/24 05:00
CRDT- 2003/11/05 05:00
PHST- 2003/11/05 05:00 [pubmed]
PHST- 2004/04/24 05:00 [medline]
PHST- 2003/11/05 05:00 [entrez]
AID - S0021-9258(18)52617-X [pii]
AID - 10.1074/jbc.M307698200 [doi]
PST - ppublish
SO  - J Biol Chem. 2004 Jan 23;279(4):2535-43. doi: 10.1074/jbc.M307698200. Epub 2003 
      Nov 3.