PMID- 14599498
OWN - NLM
STAT- MEDLINE
DCOM- 20040310
LR  - 20191026
IS  - 0965-1748 (Print)
IS  - 0965-1748 (Linking)
VI  - 33
IP  - 12
DP  - 2003 Dec
TI  - Juvenile hormone (JH) esterase: why are you so JH specific?
PG  - 1261-73
AB  - Juvenile hormone esterases (JHEs) from six insects belonging to three orders 
      (Lepidoptera, Coleoptera, and Diptera) were compared in terms of their deduced 
      amino acid sequence and biochemical properties. The four lepidopteran JHEs showed 
      from 52% to 59% identity to each other and about 30% identity to the coleopteran 
      and dipteran JHEs. The JHE of Manduca sexta was remarkably resistant to the 
      addition of organic co-solvents and detergent; in some cases, it demonstrated 
      significant activation of activity. Trifluoromethylketone (TFK) inhibitors with 
      chain lengths of 8, 10 or 12 carbons were highly effective against both 
      lepidopteran and coleopteran JHEs. The coleopteran JHE remained sensitive to TFK 
      inhibitors with a chain length of 6 carbons, whereas the lepidopteran JHEs were 
      significantly less sensitive. When the chain was altered to a phenethyl moiety, 
      the coleopteran JHE remained moderately sensitive, while the lepidopteran JHEs 
      were much less sensitive. The lepidopteran and coleopteran JHEs did not show 
      dramatic differences in specificity to alpha-naphthyl and rho-nitrophenyl 
      substrates. However, as the chain length of the alpha-naphthyl substrates 
      increased from propionate to caprylate, there was a trend towards reduced 
      activity. The JHE of M. sexta was crystallized and the properties of the crystal 
      suggest a high-resolution structure will follow.
FAU - Kamita, Shizuo G
AU  - Kamita SG
AD  - Department of Entomology and Cancer Research Center, University of California, 
      303 Briggs Hall, 1 Shields Avenue, Davis, CA 95616, USA.
FAU - Hinton, Andrew C
AU  - Hinton AC
FAU - Wheelock, Craig E
AU  - Wheelock CE
FAU - Wogulis, Mark D
AU  - Wogulis MD
FAU - Wilson, David K
AU  - Wilson DK
FAU - Wolf, Nicola M
AU  - Wolf NM
FAU - Stok, Jeanette E
AU  - Stok JE
FAU - Hock, Bertold
AU  - Hock B
FAU - Hammock, Bruce D
AU  - Hammock BD
LA  - eng
GR  - P30 ES05707/ES/NIEHS NIH HHS/United States
GR  - T32 DK07355-22/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - England
TA  - Insect Biochem Mol Biol
JT  - Insect biochemistry and molecular biology
JID - 9207282
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Oligopeptides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Sesquiterpenes)
RN  - 0 (Solvents)
RN  - B74U6BJ6J5 (juvenile hormone III)
RN  - EC 3.1.1.- (Carboxylic Ester Hydrolases)
RN  - EC 3.1.1.- (juvenile hormone esterase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carboxylic Ester Hydrolases/antagonists & inhibitors/genetics/*metabolism
MH  - Coleoptera/enzymology
MH  - Diptera/enzymology
MH  - Enzyme Inhibitors/pharmacology
MH  - Lepidoptera/enzymology
MH  - Molecular Sequence Data
MH  - Oligopeptides/chemistry/pharmacology
MH  - Recombinant Proteins/antagonists & inhibitors/chemistry/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Sesquiterpenes/*metabolism
MH  - Solvents/pharmacology
MH  - Substrate Specificity
RF  - 58
EDAT- 2003/11/06 05:00
MHDA- 2004/03/11 05:00
CRDT- 2003/11/06 05:00
PHST- 2003/11/06 05:00 [pubmed]
PHST- 2004/03/11 05:00 [medline]
PHST- 2003/11/06 05:00 [entrez]
AID - S0965174803001516 [pii]
AID - 10.1016/j.ibmb.2003.08.004 [doi]
PST - ppublish
SO  - Insect Biochem Mol Biol. 2003 Dec;33(12):1261-73. doi: 
      10.1016/j.ibmb.2003.08.004.