PMID- 14599674
OWN - NLM
STAT- MEDLINE
DCOM- 20040204
LR  - 20191210
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 114
IP  - 1
DP  - 2003 Dec
TI  - Virus concentration using polyethyleneimine-conjugated magnetic beads for 
      improving the sensitivity of nucleic acid amplification tests.
PG  - 11-9
AB  - To enhance the sensitivity of virus detection by polymerase chain reaction (PCR) 
      and reverse-transcriptional (RT)-PCR, we developed a novel virus concentration 
      method using polyethyleneimine (PEI)-conjugated magnetic beads. PEI-magnetic 
      beads adsorbed efficiently the enveloped viruses Sindbis virus and Herpes simplex 
      1 virus, and the nonenveloped virus SV-40, but not the nonenveloped viruses 
      porcine parvovirus (PPV) or poliovirus, based on the PCR detection data. 
      Furthermore, the infectivity in the supernatant of former viruses was reduced 
      markedly after incubation with PEI-magnetic beads. Both real-time PCR and RT-PCR 
      revealed that the DNA viruses were concentrated to a maximum of about 100 times 
      the expected value, whereas the RNA viruses were concentrated over a thousand 
      times, which was significantly more than expected. It was concluded that the 
      PEI-magnetic beads are a superior novel means of concentrating viruses, with the 
      exception of some non-enveloped viruses. The present method was found to enhance 
      the sensitivity of virus detection by PCR and RT-PCR.
FAU - Satoh, Koei
AU  - Satoh K
AD  - The Institute of Saitama Red Cross Center, Kamiochiai 8-3-41, Saitama 338-0001, 
      Japan.
FAU - Iwata, Akiko
AU  - Iwata A
FAU - Murata, Mitsuhiro
AU  - Murata M
FAU - Hikata, Mikio
AU  - Hikata M
FAU - Hayakawa, Takao
AU  - Hayakawa T
FAU - Yamaguchi, Teruhide
AU  - Yamaguchi T
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 9002-98-6 (Polyethyleneimine)
SB  - IM
MH  - Animals
MH  - Genome, Viral
MH  - Humans
MH  - Immunomagnetic Separation/*methods
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - *Polyethyleneimine
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Viruses/*isolation & purification
EDAT- 2003/11/06 05:00
MHDA- 2004/02/05 05:00
CRDT- 2003/11/06 05:00
PHST- 2003/11/06 05:00 [pubmed]
PHST- 2004/02/05 05:00 [medline]
PHST- 2003/11/06 05:00 [entrez]
AID - S0166093403002581 [pii]
AID - 10.1016/j.jviromet.2003.08.002 [doi]
PST - ppublish
SO  - J Virol Methods. 2003 Dec;114(1):11-9. doi: 10.1016/j.jviromet.2003.08.002.