PMID- 14600285
OWN - NLM
STAT- MEDLINE
DCOM- 20040831
LR  - 20200304
IS  - 1096-6080 (Print)
IS  - 1096-0929 (Linking)
VI  - 77
IP  - 1
DP  - 2004 Jan
TI  - The effect of chlorpyrifos and chlorpyrifos-oxon on brain cholinesterase, 
      muscarinic receptor binding, and neurotrophin levels in rats following early 
      postnatal exposure.
PG  - 63-71
AB  - Chlorpyrifos (CPS) is a widely used diethyl organophosphorus insecticide in 
      agricultural settings. Household and urinary residue analysis has suggested that 
      children in agricultural communities are at risk of exposure to diethyl 
      organophosphorus insecticides. The effects of repeated postnatal exposure to CPS 
      and its metabolite chlorpyrifos-oxon (CPO) on total muscarinic acetylcholine 
      receptor (mAChR) binding, nerve growth factor (NGF) levels, and brain derived 
      neurotrophic factor (BDNF) levels in the forebrain of neonatal rats were 
      investigated. Peak inhibition of brain cholinesterase (ChE) for CPS and CPO was 
      determined after acute exposure to dosages of each compound (a low and a high for 
      each), which produced similar degrees of initial ChE inhibition. Pups were 
      administered CPS (1.5 or 3.0 mg/kg), CPO (0.25 or 0.35 mg/kg), or the corn oil 
      vehicle by daily gavage from postnatal day 1 (PND 1) through PND 6. This exposure 
      paradigm resulted in persistent ChE inhibition by CPS but only transient 
      inhibition by CPO, suggesting that, even though the initial ChE inhibition is 
      similar between compounds, the effects of repeated exposure differ significantly. 
      Forebrain mAChR density, as measured by the binding of 3H-QNB, and NGF levels 
      were significantly reduced on PND 4 and 7 after CPS but not on PND 12. No effects 
      on mAChR density or NGF levels were observed with CPO. No effects on BDNF levels 
      were observed with either compound. The data suggest that the persistent ChE 
      inhibition and decreased mAChR binding may play a role in the decreased NGF 
      levels following CPS exposure.
FAU - Betancourt, Angela M
AU  - Betancourt AM
AD  - Center for Environmental Health Sciences, College of Veterinary Medicine, 
      Mississippi State University, Mississippi State, Mississippi 39762, USA.
FAU - Carr, Russell L
AU  - Carr RL
LA  - eng
GR  - R01 ES010386/ES/NIEHS NIH HHS/United States
GR  - 1 R01 ES 10386-01A1/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20031104
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Insecticides)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Receptors, Muscarinic)
RN  - 5598-15-2 (O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate)
RN  - EC 3.1.1.8 (Cholinesterases)
RN  - JCS58I644W (Chlorpyrifos)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Animals, Newborn
MH  - Chlorpyrifos/administration & dosage/*analogs & derivatives/*toxicity
MH  - Cholinesterases/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Insecticides/administration & dosage/*toxicity
MH  - Male
MH  - Nerve Growth Factors/*metabolism
MH  - Prosencephalon/*drug effects/growth & development/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Muscarinic/*metabolism
EDAT- 2003/11/06 05:00
MHDA- 2004/09/01 05:00
CRDT- 2003/11/06 05:00
PHST- 2003/11/06 05:00 [pubmed]
PHST- 2004/09/01 05:00 [medline]
PHST- 2003/11/06 05:00 [entrez]
AID - kfh003 [pii]
AID - 10.1093/toxsci/kfh003 [doi]
PST - ppublish
SO  - Toxicol Sci. 2004 Jan;77(1):63-71. doi: 10.1093/toxsci/kfh003. Epub 2003 Nov 4.